@article{39a52734adab4acaa018ef0752ce1ddd,
title = "Generation of two induced pluripotent stem cell lines from individuals without auditory disorders",
abstract = "We report the establishment of two human induced pluripotent stem cell (iPSC) lines from individuals without auditory disorders. Extensive audiometry tests were performed to confirm normal hearing. The generated iPSC lines expressed pluripotency genes and showed differentiation capability into the three germ layers. The iPSC lines will be used as controls for pathological analysis and drug screening for ear disorders.",
author = "Shota Okura and Honoka Ishii and Ayano Suzuki and Chika Saegusa and Ko Fujiki and Kenshi Sugano and Noriomi Suzuki and Tsubasa Saeki and Saeko Matsuzaki and Hiroyuki Ozawa and Masato Fujioka and Makoto Hosoya and Hideyuki Okano",
note = "Funding Information: We are grateful to Asako Yoshida, Takanori Nishiyama, Akifumi Mizukoshi (Kyoto University), Yuka Hiroi, and Fujiko Tsuruta for technical support and Dr. Bradley Welling (Massachusetts Eye and Ear, Boston, MA) and Kaori Kaseda for the audiological tests. The induction method for otic progenitor cells from hiPSCs was established in collaboration with Dr. Kanako Watanabe and Chihiro Mitsushima (Astellas Pharma Inc.). We thank Dr. Kazuaki Nagao for kind gift of PCR primers. We are also thankful to Drs. Hironobu Okuno, Wado Akamatsu, and Mitsuru Ishikawa for valuable suggestions and technical comments, and to all the laboratory members of H.O. for their encouragement and generous support for the current study. This study was supported by the Research Center Network for Realization of Regenerative Medicine by AMED Japan under the grants JP21bm0204001 to H.O., Grant-in-Aid for Scientific Research (A) 18H04065 and 19H05473 (to M.F. and H.O.), Grant-in-Aid for Challenging Research (Exploratory) 21K19581 (to M.F.), Grant-in-Aid for Research Activity Start-up 19K24038 (to C.S.), Grant-in-Aid for Young Scientists (B) 19K18816 and Grant-in-Aid for Scientific Research (C) 21K09660 (to S.M.) by MEXT KAKENHI, Grant-in-Aid for JSPS Fellows (20J12301 to T.S.) from Japan Society for the Promotion of Science (JSPS), the Doctoral Student Grant-in-Aid Program from Keio University (to T.S.), grant from Keio University Global Research Institute (KGRI) (to H.O.) and the Takeda Science Foundation (to M.F. and M.H). Funding Information: We are grateful to Asako Yoshida, Takanori Nishiyama, Akifumi Mizukoshi (Kyoto University), Yuka Hiroi, and Fujiko Tsuruta for technical support and Dr. Bradley Welling (Massachusetts Eye and Ear, Boston, MA) and Kaori Kaseda for the audiological tests. The induction method for otic progenitor cells from hiPSCs was established in collaboration with Dr. Kanako Watanabe and Chihiro Mitsushima (Astellas Pharma Inc.). We thank Dr. Kazuaki Nagao for kind gift of PCR primers. We are also thankful to Drs. Hironobu Okuno, Wado Akamatsu, and Mitsuru Ishikawa for valuable suggestions and technical comments, and to all the laboratory members of H.O. for their encouragement and generous support for the current study. This study was supported by the Research Center Network for Realization of Regenerative Medicine by AMED Japan under the grants JP21bm0204001 to H.O. Grant-in-Aid for Scientific Research (A) 18H04065 and 19H05473 (to M.F. and H.O.), Grant-in-Aid for Challenging Research (Exploratory) 21K19581 (to M.F.), Grant-in-Aid for Research Activity Start-up 19K24038 (to C.S.), Grant-in-Aid for Young Scientists (B) 19K18816 and Grant-in-Aid for Scientific Research (C) 21K09660 (to S.M.) by MEXT KAKENHI, Grant-in-Aid for JSPS Fellows (20J12301 to T.S.) from Japan Society for the Promotion of Science (JSPS), the Doctoral Student Grant-in-Aid Program from Keio University (to T.S.), grant from Keio University Global Research Institute (KGRI) (to H.O.) and the Takeda Science Foundation (to M.F. and M.H). Publisher Copyright: {\textcopyright} 2023",
year = "2023",
month = mar,
doi = "10.1016/j.scr.2023.103017",
language = "English",
volume = "67",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
}