Genes and molecular pathways related to radioresistance of oral squamous cell carcinoma cells

Takashi Ishigami, Katsuhiro Uzawa, Morihiro Higo, Hitomi Nomura, Kengo Saito, Yoshikuni Kato, Dai Nakashima, Masashi Shiiba, Hiroki Bukawa, Hidetaka Yokoe, Tetsuya Kawata, Hisao Ito, Hideki Tanzawa

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

To identify genes associated with radioresistant oral squamous cell carcinoma (OSCC), we compared gene expression signatures between OSCC cell lines exhibiting radioresistance and cells with radiosensitivity after X-ray irradiation in a dose-dependent manner using Affymetrix GeneChip analysis with Human Genome-U133 plus 2.0 GeneChip. The microarray data identified 167 genes that were significantly overexpressed in radioresistant cells after X-ray irradiation. Among the genes identified, 40 were mapped to 3 highly significant genetic networks identified by the Ingenuity Pathway Analysis tool. Gene ontology analysis showed that cancer-related function had the highest significance. The 40 genes included 25 cancer-related genes that formed 1 network and were categorized by function into growth and proliferation, apoptosis, and adhesion. Furthermore, real-time quantitative reverse transcriptase-polymerase chain reaction showed that the mRNA expression levels of the 25 genes were higher in radioresistant cells than in radiosensitive cells in a dose-dependent manner and in a time-dependent manner. Our results suggest that the identified genes help to elucidate the molecular mechanisms of the radioresistance of OSCC and could be radiotherapeutic molecular markers for choosing the appropriate radiotherapy for this disease.

Original languageEnglish
Pages (from-to)2262-2270
Number of pages9
JournalInternational Journal of Cancer
Volume120
Issue number10
DOIs
Publication statusPublished - 2007 May 15

Keywords

  • Microarray
  • Molecular markers
  • Oral squamous cell carcinoma
  • Pathway analysis
  • Radioresistance

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Ishigami, T., Uzawa, K., Higo, M., Nomura, H., Saito, K., Kato, Y., Nakashima, D., Shiiba, M., Bukawa, H., Yokoe, H., Kawata, T., Ito, H., & Tanzawa, H. (2007). Genes and molecular pathways related to radioresistance of oral squamous cell carcinoma cells. International Journal of Cancer, 120(10), 2262-2270. https://doi.org/10.1002/ijc.22561