Genes highly expressed in the early phase of murine graft-versus-host reaction

Masatoshi Wakui, A. Yamaguchi, D. Sakurai, K. Ogasawara, T. Yokochi, N. Tsuchiya, Y. Ikeda, K. Tokunaga

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Graft-versus-host reaction (GVHR) is a complex process initiated upon allorecognition. For detection of early molecular events in GVHR, we first assessed time courses with respect to symptoms and serum interferon (IFN)-γ levels and then used the differential display method to compare gene transcript patterns during the early phase between acute lethal GVHR mice and syngeneic controls. In the GVHR mice, high expression levels of seven genes encoding the following molecules were detected: TGTP/Mg21 (an IFN-γ-related signaling molecule), vitronectin, Nedd5 (a mammalian septin), manganese superoxide dismutase, activin βC subunit, PRCC (a papillary renal cell carcinoma-associated molecule), and an uncharacterized gene corresponding to a mouse expressed sequence tag (EST). The expression levels of most genes peaked before the symptomatological onset and the peak of IFN-γ levels. Thus, gene expression monitoring may characterize the inductive process of GVHR and aid in the development of gene-based diagnostics and therapies.

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume282
Issue number1
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Graft vs. host reactions
Genes
Transplants
Interferons
Molecules
Septins
Vitronectin
Activins
Gene encoding
Expressed Sequence Tags
Gene Expression Profiling
Renal Cell Carcinoma
Gene expression
Superoxide Dismutase
Display devices
Cells
Monitoring
Serum

Keywords

  • Activin βC subunit
  • Differential display method
  • Early phase
  • Gene expression
  • Graft-versus-host reaction
  • Manganese superoxide dismutase
  • Nedd5
  • PRCC
  • TGTP/Mg21
  • Vitronectin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Wakui, M., Yamaguchi, A., Sakurai, D., Ogasawara, K., Yokochi, T., Tsuchiya, N., ... Tokunaga, K. (2001). Genes highly expressed in the early phase of murine graft-versus-host reaction. Biochemical and Biophysical Research Communications, 282(1), 200-206. https://doi.org/10.1006/bbrc.2001.4550

Genes highly expressed in the early phase of murine graft-versus-host reaction. / Wakui, Masatoshi; Yamaguchi, A.; Sakurai, D.; Ogasawara, K.; Yokochi, T.; Tsuchiya, N.; Ikeda, Y.; Tokunaga, K.

In: Biochemical and Biophysical Research Communications, Vol. 282, No. 1, 2001, p. 200-206.

Research output: Contribution to journalArticle

Wakui, M, Yamaguchi, A, Sakurai, D, Ogasawara, K, Yokochi, T, Tsuchiya, N, Ikeda, Y & Tokunaga, K 2001, 'Genes highly expressed in the early phase of murine graft-versus-host reaction', Biochemical and Biophysical Research Communications, vol. 282, no. 1, pp. 200-206. https://doi.org/10.1006/bbrc.2001.4550
Wakui, Masatoshi ; Yamaguchi, A. ; Sakurai, D. ; Ogasawara, K. ; Yokochi, T. ; Tsuchiya, N. ; Ikeda, Y. ; Tokunaga, K. / Genes highly expressed in the early phase of murine graft-versus-host reaction. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 282, No. 1. pp. 200-206.
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