TY - JOUR
T1 - Genes highly expressed in the early phase of murine graft-versus-host reaction
AU - Wakui, Masatoshi
AU - Sakurai, Daisuke
AU - Tsuchiya, Naoyuki
AU - Tokunaga, Katsushi
AU - Wakui, Masatoshi
AU - Ikeda, Yasuo
AU - Yamaguchi, Akihiro
AU - Ogasawara, Kouetsu
AU - Yokochi, Taeko
N1 - Funding Information:
A part of this work was supported by NEDO (New Energy and Industrial Technology Development Organization, Japan).
PY - 2001
Y1 - 2001
N2 - Graft-versus-host reaction (GVHR) is a complex process initiated upon allorecognition. For detection of early molecular events in GVHR, we first assessed time courses with respect to symptoms and serum interferon (IFN)-γ levels and then used the differential display method to compare gene transcript patterns during the early phase between acute lethal GVHR mice and syngeneic controls. In the GVHR mice, high expression levels of seven genes encoding the following molecules were detected: TGTP/Mg21 (an IFN-γ-related signaling molecule), vitronectin, Nedd5 (a mammalian septin), manganese superoxide dismutase, activin βC subunit, PRCC (a papillary renal cell carcinoma-associated molecule), and an uncharacterized gene corresponding to a mouse expressed sequence tag (EST). The expression levels of most genes peaked before the symptomatological onset and the peak of IFN-γ levels. Thus, gene expression monitoring may characterize the inductive process of GVHR and aid in the development of gene-based diagnostics and therapies.
AB - Graft-versus-host reaction (GVHR) is a complex process initiated upon allorecognition. For detection of early molecular events in GVHR, we first assessed time courses with respect to symptoms and serum interferon (IFN)-γ levels and then used the differential display method to compare gene transcript patterns during the early phase between acute lethal GVHR mice and syngeneic controls. In the GVHR mice, high expression levels of seven genes encoding the following molecules were detected: TGTP/Mg21 (an IFN-γ-related signaling molecule), vitronectin, Nedd5 (a mammalian septin), manganese superoxide dismutase, activin βC subunit, PRCC (a papillary renal cell carcinoma-associated molecule), and an uncharacterized gene corresponding to a mouse expressed sequence tag (EST). The expression levels of most genes peaked before the symptomatological onset and the peak of IFN-γ levels. Thus, gene expression monitoring may characterize the inductive process of GVHR and aid in the development of gene-based diagnostics and therapies.
KW - Activin βC subunit
KW - Differential display method
KW - Early phase
KW - Gene expression
KW - Graft-versus-host reaction
KW - Manganese superoxide dismutase
KW - Nedd5
KW - PRCC
KW - TGTP/Mg21
KW - Vitronectin
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U2 - 10.1006/bbrc.2001.4550
DO - 10.1006/bbrc.2001.4550
M3 - Article
C2 - 11263992
AN - SCOPUS:0034813625
VL - 282
SP - 200
EP - 206
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -