Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro

Kazuo Sugiyama, Kenji Suzuki, Takahide Nakazawa, Kenji Funami, Takayuki Hishiki, Kazuya Ogawa, Satoru Saito, Kumiko W. Shimotohno, Takeshi Suzuki, Yuko Shimizu, Reiri Tobita, Makoto Hijikata, Hiroshi Takaku, Kunitada Shimotohno

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.

Original languageEnglish
Pages (from-to)6922-6928
Number of pages7
JournalJournal of Virology
Volume83
Issue number13
DOIs
Publication statusPublished - 2009 Jul 1

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Sugiyama, K., Suzuki, K., Nakazawa, T., Funami, K., Hishiki, T., Ogawa, K., Saito, S., Shimotohno, K. W., Suzuki, T., Shimizu, Y., Tobita, R., Hijikata, M., Takaku, H., & Shimotohno, K. (2009). Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. Journal of Virology, 83(13), 6922-6928. https://doi.org/10.1128/JVI.02674-08