Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro

Kazuo Sugiyama, Kenji Suzuki, Takahide Nakazawa, Kenji Funami, Takayuki Hishiki, Kazuya Ogawa, Satoru Saito, Kumiko W. Shimotohno, Takeshi Suzuki, Yuko Shimizu, Reiri Tobita, Makoto Hijikata, Hiroshi Takaku, Kunitada Shimotohno

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.

Original languageEnglish
Pages (from-to)6922-6928
Number of pages7
JournalJournal of Virology
Volume83
Issue number13
DOIs
Publication statusPublished - 2009 Jul

Fingerprint

Hepatitis C virus
Hepacivirus
genetic techniques and protocols
pathogenicity
Genome
genome
blood serum
structural proteins
Serum
In Vitro Techniques
phylogeny
blood
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Sugiyama, K., Suzuki, K., Nakazawa, T., Funami, K., Hishiki, T., Ogawa, K., ... Shimotohno, K. (2009). Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. Journal of Virology, 83(13), 6922-6928. https://doi.org/10.1128/JVI.02674-08

Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. / Sugiyama, Kazuo; Suzuki, Kenji; Nakazawa, Takahide; Funami, Kenji; Hishiki, Takayuki; Ogawa, Kazuya; Saito, Satoru; Shimotohno, Kumiko W.; Suzuki, Takeshi; Shimizu, Yuko; Tobita, Reiri; Hijikata, Makoto; Takaku, Hiroshi; Shimotohno, Kunitada.

In: Journal of Virology, Vol. 83, No. 13, 07.2009, p. 6922-6928.

Research output: Contribution to journalArticle

Sugiyama, K, Suzuki, K, Nakazawa, T, Funami, K, Hishiki, T, Ogawa, K, Saito, S, Shimotohno, KW, Suzuki, T, Shimizu, Y, Tobita, R, Hijikata, M, Takaku, H & Shimotohno, K 2009, 'Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro', Journal of Virology, vol. 83, no. 13, pp. 6922-6928. https://doi.org/10.1128/JVI.02674-08
Sugiyama K, Suzuki K, Nakazawa T, Funami K, Hishiki T, Ogawa K et al. Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. Journal of Virology. 2009 Jul;83(13):6922-6928. https://doi.org/10.1128/JVI.02674-08
Sugiyama, Kazuo ; Suzuki, Kenji ; Nakazawa, Takahide ; Funami, Kenji ; Hishiki, Takayuki ; Ogawa, Kazuya ; Saito, Satoru ; Shimotohno, Kumiko W. ; Suzuki, Takeshi ; Shimizu, Yuko ; Tobita, Reiri ; Hijikata, Makoto ; Takaku, Hiroshi ; Shimotohno, Kunitada. / Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro. In: Journal of Virology. 2009 ; Vol. 83, No. 13. pp. 6922-6928.
@article{2b0796e5e57b47a094d6723ffe072b30,
title = "Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro",
abstract = "Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.",
author = "Kazuo Sugiyama and Kenji Suzuki and Takahide Nakazawa and Kenji Funami and Takayuki Hishiki and Kazuya Ogawa and Satoru Saito and Shimotohno, {Kumiko W.} and Takeshi Suzuki and Yuko Shimizu and Reiri Tobita and Makoto Hijikata and Hiroshi Takaku and Kunitada Shimotohno",
year = "2009",
month = "7",
doi = "10.1128/JVI.02674-08",
language = "English",
volume = "83",
pages = "6922--6928",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "13",

}

TY - JOUR

T1 - Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro

AU - Sugiyama, Kazuo

AU - Suzuki, Kenji

AU - Nakazawa, Takahide

AU - Funami, Kenji

AU - Hishiki, Takayuki

AU - Ogawa, Kazuya

AU - Saito, Satoru

AU - Shimotohno, Kumiko W.

AU - Suzuki, Takeshi

AU - Shimizu, Yuko

AU - Tobita, Reiri

AU - Hijikata, Makoto

AU - Takaku, Hiroshi

AU - Shimotohno, Kunitada

PY - 2009/7

Y1 - 2009/7

N2 - Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.

AB - Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.

UR - http://www.scopus.com/inward/record.url?scp=67449099426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67449099426&partnerID=8YFLogxK

U2 - 10.1128/JVI.02674-08

DO - 10.1128/JVI.02674-08

M3 - Article

C2 - 19369330

AN - SCOPUS:67449099426

VL - 83

SP - 6922

EP - 6928

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 13

ER -