Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro

Kazuo Sugiyama; Kenji Suzuki; Takahide Nakazawa; Kenji Funami; Takayuki Hishiki; Kazuya Ogawa; Satoru Saito; Kumiko W. Shimotohno; Takeshi Suzuki; Yuko Shimizu; Reiri Tobita; Makoto Hijikata; Hiroshi Takaku; Kunitada Shimotohno

doi:10.1128/JVI.02674-08

Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro

Kazuo Sugiyama, Kenji Suzuki, Takahide Nakazawa, Kenji Funami, Takayuki Hishiki, Kazuya Ogawa, Satoru Saito, Kumiko W. Shimotohno, Takeshi Suzuki, Yuko Shimizu, Reiri Tobita, Makoto Hijikata, Hiroshi Takaku, Kunitada Shimotohno

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.

Original language	English
Pages (from-to)	6922-6928
Number of pages	7
Journal	Journal of Virology
Volume	83
Issue number	13
DOIs	https://doi.org/10.1128/JVI.02674-08
Publication status	Published - 2009 Jul

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JVI.02674-08

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abstract = "Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.",

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AU - Sugiyama, Kazuo

AU - Suzuki, Kenji

AU - Nakazawa, Takahide

AU - Funami, Kenji

AU - Hishiki, Takayuki

AU - Ogawa, Kazuya

AU - Saito, Satoru

AU - Shimotohno, Kumiko W.

AU - Suzuki, Takeshi

AU - Shimizu, Yuko

AU - Tobita, Reiri

AU - Hijikata, Makoto

AU - Takaku, Hiroshi

AU - Shimotohno, Kunitada

PY - 2009/7

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AB - Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.

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Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro

Abstract

ASJC Scopus subject areas

UN SDGs

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