TY - JOUR
T1 - Genetic analysis of salt-marsh sedge Carex scabrifolia Steud. populations using newly developed microsatellite markers
AU - Hodoki, Yoshikuni
AU - Ohbayashi, Kako
AU - Kunii, Hidenobu
N1 - Funding Information:
Acknowledgments This study was supported by grants from the Japan Society for the Promotion of Science (Grant-in-Aid for Scientific Research (A) (19201017)) and Foundation for Riverfront Improvement and Restoration.
PY - 2009/9
Y1 - 2009/9
N2 - Nine microsatellite loci were isolated and characterized from the clonal salt-marsh sedge Carex scabrifolia, and genetic diversities within four populations were analyzed. The number of alleles per locus ranged from 2 to 7, with an average of 4.7. The observed and expected heterozygosities ranged from 0.000 to 1.000 and 0.000 to 0.679, respectively. In two populations, almost all polymorphic loci showed a significant excess of heterozygotes due to their high clonal reproduction and low number of genotypes (genets) composing the populations. Thus, the number of identical genets varied greatly among populations and ranged from 1 to 28, irrespective of population size (i.e., population area and ramet number). When attempting to conserve and restore clonal plants such as C. scabrifolia, it is important to preserve sufficient genetic diversity within a population, which can be assessed using genetic markers such as the simple sequence repeat markers described here.
AB - Nine microsatellite loci were isolated and characterized from the clonal salt-marsh sedge Carex scabrifolia, and genetic diversities within four populations were analyzed. The number of alleles per locus ranged from 2 to 7, with an average of 4.7. The observed and expected heterozygosities ranged from 0.000 to 1.000 and 0.000 to 0.679, respectively. In two populations, almost all polymorphic loci showed a significant excess of heterozygotes due to their high clonal reproduction and low number of genotypes (genets) composing the populations. Thus, the number of identical genets varied greatly among populations and ranged from 1 to 28, irrespective of population size (i.e., population area and ramet number). When attempting to conserve and restore clonal plants such as C. scabrifolia, it is important to preserve sufficient genetic diversity within a population, which can be assessed using genetic markers such as the simple sequence repeat markers described here.
KW - Carex scabrifolia
KW - Compound microsatellite marker
KW - Salt-marsh sedge
KW - Simple sequence repeat
UR - http://www.scopus.com/inward/record.url?scp=70350546295&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350546295&partnerID=8YFLogxK
U2 - 10.1007/s10592-008-9678-y
DO - 10.1007/s10592-008-9678-y
M3 - Article
AN - SCOPUS:70350546295
SN - 1566-0621
VL - 10
SP - 1361
EP - 1364
JO - Conservation Genetics
JF - Conservation Genetics
IS - 5
ER -