Genetic analysis of salt-marsh sedge Carex scabrifolia Steud. populations using newly developed microsatellite markers

Yoshikuni Hodoki, Kako Ohbayashi, Hidenobu Kunii

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Nine microsatellite loci were isolated and characterized from the clonal salt-marsh sedge Carex scabrifolia, and genetic diversities within four populations were analyzed. The number of alleles per locus ranged from 2 to 7, with an average of 4.7. The observed and expected heterozygosities ranged from 0.000 to 1.000 and 0.000 to 0.679, respectively. In two populations, almost all polymorphic loci showed a significant excess of heterozygotes due to their high clonal reproduction and low number of genotypes (genets) composing the populations. Thus, the number of identical genets varied greatly among populations and ranged from 1 to 28, irrespective of population size (i.e., population area and ramet number). When attempting to conserve and restore clonal plants such as C. scabrifolia, it is important to preserve sufficient genetic diversity within a population, which can be assessed using genetic markers such as the simple sequence repeat markers described here.

Original languageEnglish
Pages (from-to)1361-1364
Number of pages4
JournalConservation Genetics
Volume10
Issue number5
DOIs
Publication statusPublished - 2009 Sep
Externally publishedYes

Fingerprint

genet
sedge
Wetlands
Carex
genetic analysis
salt marshes
saltmarsh
Microsatellite Repeats
genetic techniques and protocols
Salts
microsatellite repeats
clonal organism
ramet
genetic marker
heterozygosity
Population
Viverridae
population size
allele
genotype

Keywords

  • Carex scabrifolia
  • Compound microsatellite marker
  • Salt-marsh sedge
  • Simple sequence repeat

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Genetic analysis of salt-marsh sedge Carex scabrifolia Steud. populations using newly developed microsatellite markers. / Hodoki, Yoshikuni; Ohbayashi, Kako; Kunii, Hidenobu.

In: Conservation Genetics, Vol. 10, No. 5, 09.2009, p. 1361-1364.

Research output: Contribution to journalArticle

Hodoki, Yoshikuni ; Ohbayashi, Kako ; Kunii, Hidenobu. / Genetic analysis of salt-marsh sedge Carex scabrifolia Steud. populations using newly developed microsatellite markers. In: Conservation Genetics. 2009 ; Vol. 10, No. 5. pp. 1361-1364.
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