Genetic analysis of vertebral regionalization and number in medaka (oryzias latipes) inbred lines

Tetsuaki Kimura, Minori Shinya, Kiyosi Naruse

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Vertebral number is the most variable trait among vertebrates. In addition to the vertebral number, the ratio of abdominal to caudal vertebrae is a variable trait. The vertebral number and the ratio of abdominal to caudal vertebrae contribute to vertebrate diversity. It is very interesting to know how to determine the vertebral number and the ratio of abdominal to caudal vertebrae. In this study, we identify differences in the vertebral number and the ratio of abdominal vertebrae to vertebral number between two inbred lines of medaka, namely, Hd-rRII1 and Kaga. To identify the genetic factor of those differences, we performed quantitative trait locus (QTL) analysis for vertebral number and the ratio of abdominal vertebrae to vertebral number using 200 F2 fish. Our results show a suggestive QTL of the ratio of abdominal vertebrae to vertebral number on chromosome 15, and five QTL of vertebral number on chromosomes 1, 10, 11, 17, and 23. The QTL on chromosome 15 contains hoxDb cluster genes. The QTL of vertebral number include some genes related to the segmentation clock and axial elongation. In addition, we show that the difference in vertebral number between two inbred lines is derived from differences in the anteroposterior length of somites. Our results emphasize that the developmental process should be considered in genetic analyses for vertebral number.

Original languageEnglish
Pages (from-to)1317-1323
Number of pages7
JournalG3: Genes, Genomes, Genetics
Volume2
Issue number11
DOIs
Publication statusPublished - 2012 Nov
Externally publishedYes

Fingerprint

Oryzias
Quantitative Trait Loci
Spine
Chromosomes, Human, Pair 15
Vertebrates
Chromosomes, Human, Pair 10
Somites
Chromosomes, Human, Pair 1
Multigene Family
Fishes
Genes

Keywords

  • Abdominal
  • Caudal vertebrae
  • Genome-wide
  • Marker set
  • Vertebrae
  • Vertebral number

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Genetic analysis of vertebral regionalization and number in medaka (oryzias latipes) inbred lines. / Kimura, Tetsuaki; Shinya, Minori; Naruse, Kiyosi.

In: G3: Genes, Genomes, Genetics, Vol. 2, No. 11, 11.2012, p. 1317-1323.

Research output: Contribution to journalArticle

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