Genetic and chemical comparison of Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii)

Tomoko Sano, Ikue Matsumura, Rie Nakamura, Hiroki Yamaji, Kazunori Hashimoto, Osami Takeda, Fumiyuki Kiuchi, Tadahiro Takeda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Boi and its original plant Sinomenium acutum from Japan were compared with Seifuto and its botanical origins from China in terms of their internal transcribed spacer (ITS) sequences and major chemical components. Boi, Seifuto, and their botanical origins overall showed seven variable sites in the ITS sequence and six genotypes. Japanese S. acutum and Boi had one nucleotide variation at position 593 to show two genotypes (J1 and J2) and their heterozygote (J3). Seifuto samples and their botanical origins, S. acutum and S. acutum var. cinereum from China, showed three genotypes (C1, C2, and C3), which did not agree with the botanical classification, indicating that they cannot be distinguished according to their ITS sequences. All Seifuto samples from Henan market showed the same ITS genotype (C1). The Japanese and Chinese genotypes differed in the nucleotide position 424, which can be used to distinguish the country of origin of these materials. In the HPLC analysis of six major components, sinomenine (1), magnoflorine (2), menisperine (3), 6-O-methyllaudanosoline glucoside (4), liriodendrin (5), and menisdaurin (6), all were detected in Boi, whereas five (all except for menisdaurin) were detected in Seifuto. The main component in the rhizome of Seifuto was sinomenine, whereas magnoflorine was the main component in the rhizome and the climbing stem of Boi. The content of sinomenine in Seifuto was almost twice that in Boi. Although the individual content of alkaloids 1-4 differed between Boi and Seifuto, the total contents of these alkaloids were comparable between them both in the climbing stem and rhizome.

Original languageEnglish
Pages (from-to)257-265
Number of pages9
JournalJournal of Natural Medicines
Volume64
Issue number3
DOIs
Publication statusPublished - 2010 Jul

Fingerprint

Sinomenium
Genotype
Rhizome
Alkaloids
Nucleotides
Glucosides
China
Heterozygote
Japan
High Pressure Liquid Chromatography
sinomenine
magnoflorine
menisdaurin

Keywords

  • Alkaloid
  • Boi
  • Internal transcribed spacer (ITS) regions
  • Seifuto
  • Sinomenine
  • Sinomenium acutum

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Complementary and alternative medicine

Cite this

Sano, T., Matsumura, I., Nakamura, R., Yamaji, H., Hashimoto, K., Takeda, O., ... Takeda, T. (2010). Genetic and chemical comparison of Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii). Journal of Natural Medicines, 64(3), 257-265. https://doi.org/10.1007/s11418-010-0397-6

Genetic and chemical comparison of Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii). / Sano, Tomoko; Matsumura, Ikue; Nakamura, Rie; Yamaji, Hiroki; Hashimoto, Kazunori; Takeda, Osami; Kiuchi, Fumiyuki; Takeda, Tadahiro.

In: Journal of Natural Medicines, Vol. 64, No. 3, 07.2010, p. 257-265.

Research output: Contribution to journalArticle

Sano, T, Matsumura, I, Nakamura, R, Yamaji, H, Hashimoto, K, Takeda, O, Kiuchi, F & Takeda, T 2010, 'Genetic and chemical comparison of Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii)', Journal of Natural Medicines, vol. 64, no. 3, pp. 257-265. https://doi.org/10.1007/s11418-010-0397-6
Sano, Tomoko ; Matsumura, Ikue ; Nakamura, Rie ; Yamaji, Hiroki ; Hashimoto, Kazunori ; Takeda, Osami ; Kiuchi, Fumiyuki ; Takeda, Tadahiro. / Genetic and chemical comparison of Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii). In: Journal of Natural Medicines. 2010 ; Vol. 64, No. 3. pp. 257-265.
@article{e22243bf925c4a778c5939fcf2eac1a4,
title = "Genetic and chemical comparison of Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii)",
abstract = "Boi and its original plant Sinomenium acutum from Japan were compared with Seifuto and its botanical origins from China in terms of their internal transcribed spacer (ITS) sequences and major chemical components. Boi, Seifuto, and their botanical origins overall showed seven variable sites in the ITS sequence and six genotypes. Japanese S. acutum and Boi had one nucleotide variation at position 593 to show two genotypes (J1 and J2) and their heterozygote (J3). Seifuto samples and their botanical origins, S. acutum and S. acutum var. cinereum from China, showed three genotypes (C1, C2, and C3), which did not agree with the botanical classification, indicating that they cannot be distinguished according to their ITS sequences. All Seifuto samples from Henan market showed the same ITS genotype (C1). The Japanese and Chinese genotypes differed in the nucleotide position 424, which can be used to distinguish the country of origin of these materials. In the HPLC analysis of six major components, sinomenine (1), magnoflorine (2), menisperine (3), 6-O-methyllaudanosoline glucoside (4), liriodendrin (5), and menisdaurin (6), all were detected in Boi, whereas five (all except for menisdaurin) were detected in Seifuto. The main component in the rhizome of Seifuto was sinomenine, whereas magnoflorine was the main component in the rhizome and the climbing stem of Boi. The content of sinomenine in Seifuto was almost twice that in Boi. Although the individual content of alkaloids 1-4 differed between Boi and Seifuto, the total contents of these alkaloids were comparable between them both in the climbing stem and rhizome.",
keywords = "Alkaloid, Boi, Internal transcribed spacer (ITS) regions, Seifuto, Sinomenine, Sinomenium acutum",
author = "Tomoko Sano and Ikue Matsumura and Rie Nakamura and Hiroki Yamaji and Kazunori Hashimoto and Osami Takeda and Fumiyuki Kiuchi and Tadahiro Takeda",
year = "2010",
month = "7",
doi = "10.1007/s11418-010-0397-6",
language = "English",
volume = "64",
pages = "257--265",
journal = "Journal of Natural Medicines",
issn = "1861-0293",
publisher = "Springer Japan",
number = "3",

}

TY - JOUR

T1 - Genetic and chemical comparison of Boi (Sinomeni Caulis et Rhizoma) and Seifuto (Caulis Sinomenii)

AU - Sano, Tomoko

AU - Matsumura, Ikue

AU - Nakamura, Rie

AU - Yamaji, Hiroki

AU - Hashimoto, Kazunori

AU - Takeda, Osami

AU - Kiuchi, Fumiyuki

AU - Takeda, Tadahiro

PY - 2010/7

Y1 - 2010/7

N2 - Boi and its original plant Sinomenium acutum from Japan were compared with Seifuto and its botanical origins from China in terms of their internal transcribed spacer (ITS) sequences and major chemical components. Boi, Seifuto, and their botanical origins overall showed seven variable sites in the ITS sequence and six genotypes. Japanese S. acutum and Boi had one nucleotide variation at position 593 to show two genotypes (J1 and J2) and their heterozygote (J3). Seifuto samples and their botanical origins, S. acutum and S. acutum var. cinereum from China, showed three genotypes (C1, C2, and C3), which did not agree with the botanical classification, indicating that they cannot be distinguished according to their ITS sequences. All Seifuto samples from Henan market showed the same ITS genotype (C1). The Japanese and Chinese genotypes differed in the nucleotide position 424, which can be used to distinguish the country of origin of these materials. In the HPLC analysis of six major components, sinomenine (1), magnoflorine (2), menisperine (3), 6-O-methyllaudanosoline glucoside (4), liriodendrin (5), and menisdaurin (6), all were detected in Boi, whereas five (all except for menisdaurin) were detected in Seifuto. The main component in the rhizome of Seifuto was sinomenine, whereas magnoflorine was the main component in the rhizome and the climbing stem of Boi. The content of sinomenine in Seifuto was almost twice that in Boi. Although the individual content of alkaloids 1-4 differed between Boi and Seifuto, the total contents of these alkaloids were comparable between them both in the climbing stem and rhizome.

AB - Boi and its original plant Sinomenium acutum from Japan were compared with Seifuto and its botanical origins from China in terms of their internal transcribed spacer (ITS) sequences and major chemical components. Boi, Seifuto, and their botanical origins overall showed seven variable sites in the ITS sequence and six genotypes. Japanese S. acutum and Boi had one nucleotide variation at position 593 to show two genotypes (J1 and J2) and their heterozygote (J3). Seifuto samples and their botanical origins, S. acutum and S. acutum var. cinereum from China, showed three genotypes (C1, C2, and C3), which did not agree with the botanical classification, indicating that they cannot be distinguished according to their ITS sequences. All Seifuto samples from Henan market showed the same ITS genotype (C1). The Japanese and Chinese genotypes differed in the nucleotide position 424, which can be used to distinguish the country of origin of these materials. In the HPLC analysis of six major components, sinomenine (1), magnoflorine (2), menisperine (3), 6-O-methyllaudanosoline glucoside (4), liriodendrin (5), and menisdaurin (6), all were detected in Boi, whereas five (all except for menisdaurin) were detected in Seifuto. The main component in the rhizome of Seifuto was sinomenine, whereas magnoflorine was the main component in the rhizome and the climbing stem of Boi. The content of sinomenine in Seifuto was almost twice that in Boi. Although the individual content of alkaloids 1-4 differed between Boi and Seifuto, the total contents of these alkaloids were comparable between them both in the climbing stem and rhizome.

KW - Alkaloid

KW - Boi

KW - Internal transcribed spacer (ITS) regions

KW - Seifuto

KW - Sinomenine

KW - Sinomenium acutum

UR - http://www.scopus.com/inward/record.url?scp=77953619741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953619741&partnerID=8YFLogxK

U2 - 10.1007/s11418-010-0397-6

DO - 10.1007/s11418-010-0397-6

M3 - Article

C2 - 20217263

AN - SCOPUS:77953619741

VL - 64

SP - 257

EP - 265

JO - Journal of Natural Medicines

JF - Journal of Natural Medicines

SN - 1861-0293

IS - 3

ER -