Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector

Shinya Kaneko; Takashi Takeuchi; Mitsuhiro Itaya

doi:10.1016/j.jbiotec.2008.12.007

Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector

Shinya Kaneko, Takashi Takeuchi, Mitsuhiro Itaya

Faculty of Environment and Information Studies

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.

Original language	English
Pages (from-to)	211-213
Number of pages	3
Journal	Journal of Biotechnology
Volume	139
Issue number	3
DOIs	https://doi.org/10.1016/j.jbiotec.2008.12.007
Publication status	Published - 2009 Feb 5

Keywords

Genome vector
Mouse genome
Recombination
Transformation

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiotec.2008.12.007

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abstract = "A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.",

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AB - A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.

KW - Genome vector

KW - Mouse genome

KW - Recombination

KW - Transformation

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Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector

Abstract

Keywords

ASJC Scopus subject areas

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