Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector

Shinya Kaneko, Takashi Takeuchi, Mitsuhiro Itaya

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.

Original languageEnglish
Pages (from-to)211-213
Number of pages3
JournalJournal of Biotechnology
Volume139
Issue number3
DOIs
Publication statusPublished - 2009 Feb 5

Keywords

  • Genome vector
  • Mouse genome
  • Recombination
  • Transformation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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