TY - JOUR
T1 - Genetic diagnosis by polymerase chain reaction and electrospray ionization mass spectrometry
T2 - Detection of five base deletion from blood DNA of a familial adenomatous polyposis patient
AU - Naito, Yasuhiro
AU - Ishikawa, Keiichiro
AU - Koga, Yoshinori
AU - Tsuneyoshi, Toshihiro
AU - Terunuma, Hideya
AU - Arakawa, Ryuichi
PY - 1997/7/1
Y1 - 1997/7/1
N2 - A 5-base deleted mutation of adenomatous polyposis coli (APC) gene was detected by using electrospray ionization mass spectrometry of polymerase chain reaction (PCR) products. Genomic DNA was extracted from a familial adenomatous polyposis patient blood, and a 57-base pairs segment of APC gene was amplified by PCR. The PCR products were purified, digested with restriction endonuclease, purified, and determined by electrospray mass spectrometry.
AB - A 5-base deleted mutation of adenomatous polyposis coli (APC) gene was detected by using electrospray ionization mass spectrometry of polymerase chain reaction (PCR) products. Genomic DNA was extracted from a familial adenomatous polyposis patient blood, and a 57-base pairs segment of APC gene was amplified by PCR. The PCR products were purified, digested with restriction endonuclease, purified, and determined by electrospray mass spectrometry.
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U2 - 10.1016/S1044-0305(97)00083-4
DO - 10.1016/S1044-0305(97)00083-4
M3 - Article
AN - SCOPUS:0031194993
SN - 1044-0305
VL - 8
SP - 737
EP - 742
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 7
ER -