Genetic diagnosis by polymerase chain reaction and electrospray ionization mass spectrometry

Detection of five base deletion from blood DNA of a familial adenomatous polyposis patient

Yasuhiro Naito, Keiichiro Ishikawa, Yoshinori Koga, Toshihiro Tsuneyoshi, Hideya Terunuma, Ryuichi Arakawa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A 5-base deleted mutation of adenomatous polyposis coli (APC) gene was detected by using electrospray ionization mass spectrometry of polymerase chain reaction (PCR) products. Genomic DNA was extracted from a familial adenomatous polyposis patient blood, and a 57-base pairs segment of APC gene was amplified by PCR. The PCR products were purified, digested with restriction endonuclease, purified, and determined by electrospray mass spectrometry.

Original languageEnglish
Pages (from-to)737-742
Number of pages6
JournalJournal of the American Society for Mass Spectrometry
Volume8
Issue number7
DOIs
Publication statusPublished - 1997 Jul 1
Externally publishedYes

Fingerprint

Electrospray ionization
Adenomatous Polyposis Coli
Electrospray Ionization Mass Spectrometry
Polymerase chain reaction
APC Genes
Mass spectrometry
Blood
Reaction products
Polymerase Chain Reaction
DNA
Genes
DNA Restriction Enzymes
Base Pairing
Mass Spectrometry
Mutation

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Cite this

Genetic diagnosis by polymerase chain reaction and electrospray ionization mass spectrometry : Detection of five base deletion from blood DNA of a familial adenomatous polyposis patient. / Naito, Yasuhiro; Ishikawa, Keiichiro; Koga, Yoshinori; Tsuneyoshi, Toshihiro; Terunuma, Hideya; Arakawa, Ryuichi.

In: Journal of the American Society for Mass Spectrometry, Vol. 8, No. 7, 01.07.1997, p. 737-742.

Research output: Contribution to journalArticle

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