Genetic evidence for a protective role of heat shock factor 1 against irritant-induced gastric lesions

Ken Ichiro Tanaka, Shinji Tsutsumi, Yasuhiro Arai, Tatsuya Hoshino, Keitarou Suzuki, Eiichi Takaki, Takaaki Ito, Koji Takeuchi, Akira Nakai, Tohru Mizushima

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Gastric lesions result from an imbalance between aggressive and defensive factors. Indirect lines of evidence suggest that heat shock proteins (HSPs) induced by various aggressive factors provide a major protective mechanism. In this study, we compared gastric ulcerogenic response in wild-type mice and in those lacking heat shock factor 1 (HSF1), a transcription factor for hsp genes. The severity of gastric lesions induced by ethanol or hydrochloric acid was worsened in HSF1-null mice. Immunoblotting, real-time reverse transcription-polymerase chain reaction, immunohistochemical analysis, and terminal deoxynucleotidyl transferase dUTP nick-end labeling assay revealed that the ethanol administration up-regulated gastric mucosal HSPs, in particular HSP70, in an HSF1-dependent manner, and more apoptotic cells were observed in the gastric mucosa of HSF1-null mice than in wild-type mice. In contrast, other parameters governing the gastric ulcerogenic response, including gastric acid secretion, gastric mucosal blood flow, and prostaglandin E2 levels, were not significantly affected by the absence of the hsf1 gene. Geranylgeranylacetone (GGA), a clinically used antiulcer drug with HSP-inducing activity, suppressed ethanol-induced gastric lesions in wild-type mice but not in heat shock factor 1 (HSF1)-null mice. The results suggest that the aggravation of irritant-induced gastric lesions in HSF1-null mice is due to their inability to up-regulate HSPs, leading to apoptosis. It is also suggested that the HSP-inducing activity of GGA contributes to the drug's antiulcer activity. This study provides direct genetic evidence that HSPs, after their HSF1-dependent up-regulation, confer gastric protection against the irritant-induced lesions.

Original languageEnglish
Pages (from-to)985-993
Number of pages9
JournalMolecular Pharmacology
Volume71
Issue number4
DOIs
Publication statusPublished - 2007 Apr
Externally publishedYes

Fingerprint

Irritants
Shock
Stomach
Hot Temperature
Heat-Shock Proteins
geranylgeranylacetone
Ethanol
Up-Regulation
DNA Nucleotidylexotransferase
Hydrochloric Acid
Gastric Acid
Gastric Mucosa
Dinoprostone
Immunoblotting
Pharmaceutical Preparations
Genes
Reverse Transcription
Transcription Factors
Apoptosis
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Pharmacology

Cite this

Tanaka, K. I., Tsutsumi, S., Arai, Y., Hoshino, T., Suzuki, K., Takaki, E., ... Mizushima, T. (2007). Genetic evidence for a protective role of heat shock factor 1 against irritant-induced gastric lesions. Molecular Pharmacology, 71(4), 985-993. https://doi.org/10.1124/mol.106.033282

Genetic evidence for a protective role of heat shock factor 1 against irritant-induced gastric lesions. / Tanaka, Ken Ichiro; Tsutsumi, Shinji; Arai, Yasuhiro; Hoshino, Tatsuya; Suzuki, Keitarou; Takaki, Eiichi; Ito, Takaaki; Takeuchi, Koji; Nakai, Akira; Mizushima, Tohru.

In: Molecular Pharmacology, Vol. 71, No. 4, 04.2007, p. 985-993.

Research output: Contribution to journalArticle

Tanaka, KI, Tsutsumi, S, Arai, Y, Hoshino, T, Suzuki, K, Takaki, E, Ito, T, Takeuchi, K, Nakai, A & Mizushima, T 2007, 'Genetic evidence for a protective role of heat shock factor 1 against irritant-induced gastric lesions', Molecular Pharmacology, vol. 71, no. 4, pp. 985-993. https://doi.org/10.1124/mol.106.033282
Tanaka, Ken Ichiro ; Tsutsumi, Shinji ; Arai, Yasuhiro ; Hoshino, Tatsuya ; Suzuki, Keitarou ; Takaki, Eiichi ; Ito, Takaaki ; Takeuchi, Koji ; Nakai, Akira ; Mizushima, Tohru. / Genetic evidence for a protective role of heat shock factor 1 against irritant-induced gastric lesions. In: Molecular Pharmacology. 2007 ; Vol. 71, No. 4. pp. 985-993.
@article{40fa60c5b244442198922b25c013ccfd,
title = "Genetic evidence for a protective role of heat shock factor 1 against irritant-induced gastric lesions",
abstract = "Gastric lesions result from an imbalance between aggressive and defensive factors. Indirect lines of evidence suggest that heat shock proteins (HSPs) induced by various aggressive factors provide a major protective mechanism. In this study, we compared gastric ulcerogenic response in wild-type mice and in those lacking heat shock factor 1 (HSF1), a transcription factor for hsp genes. The severity of gastric lesions induced by ethanol or hydrochloric acid was worsened in HSF1-null mice. Immunoblotting, real-time reverse transcription-polymerase chain reaction, immunohistochemical analysis, and terminal deoxynucleotidyl transferase dUTP nick-end labeling assay revealed that the ethanol administration up-regulated gastric mucosal HSPs, in particular HSP70, in an HSF1-dependent manner, and more apoptotic cells were observed in the gastric mucosa of HSF1-null mice than in wild-type mice. In contrast, other parameters governing the gastric ulcerogenic response, including gastric acid secretion, gastric mucosal blood flow, and prostaglandin E2 levels, were not significantly affected by the absence of the hsf1 gene. Geranylgeranylacetone (GGA), a clinically used antiulcer drug with HSP-inducing activity, suppressed ethanol-induced gastric lesions in wild-type mice but not in heat shock factor 1 (HSF1)-null mice. The results suggest that the aggravation of irritant-induced gastric lesions in HSF1-null mice is due to their inability to up-regulate HSPs, leading to apoptosis. It is also suggested that the HSP-inducing activity of GGA contributes to the drug's antiulcer activity. This study provides direct genetic evidence that HSPs, after their HSF1-dependent up-regulation, confer gastric protection against the irritant-induced lesions.",
author = "Tanaka, {Ken Ichiro} and Shinji Tsutsumi and Yasuhiro Arai and Tatsuya Hoshino and Keitarou Suzuki and Eiichi Takaki and Takaaki Ito and Koji Takeuchi and Akira Nakai and Tohru Mizushima",
year = "2007",
month = "4",
doi = "10.1124/mol.106.033282",
language = "English",
volume = "71",
pages = "985--993",
journal = "Molecular Pharmacology",
issn = "0026-895X",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "4",

}

TY - JOUR

T1 - Genetic evidence for a protective role of heat shock factor 1 against irritant-induced gastric lesions

AU - Tanaka, Ken Ichiro

AU - Tsutsumi, Shinji

AU - Arai, Yasuhiro

AU - Hoshino, Tatsuya

AU - Suzuki, Keitarou

AU - Takaki, Eiichi

AU - Ito, Takaaki

AU - Takeuchi, Koji

AU - Nakai, Akira

AU - Mizushima, Tohru

PY - 2007/4

Y1 - 2007/4

N2 - Gastric lesions result from an imbalance between aggressive and defensive factors. Indirect lines of evidence suggest that heat shock proteins (HSPs) induced by various aggressive factors provide a major protective mechanism. In this study, we compared gastric ulcerogenic response in wild-type mice and in those lacking heat shock factor 1 (HSF1), a transcription factor for hsp genes. The severity of gastric lesions induced by ethanol or hydrochloric acid was worsened in HSF1-null mice. Immunoblotting, real-time reverse transcription-polymerase chain reaction, immunohistochemical analysis, and terminal deoxynucleotidyl transferase dUTP nick-end labeling assay revealed that the ethanol administration up-regulated gastric mucosal HSPs, in particular HSP70, in an HSF1-dependent manner, and more apoptotic cells were observed in the gastric mucosa of HSF1-null mice than in wild-type mice. In contrast, other parameters governing the gastric ulcerogenic response, including gastric acid secretion, gastric mucosal blood flow, and prostaglandin E2 levels, were not significantly affected by the absence of the hsf1 gene. Geranylgeranylacetone (GGA), a clinically used antiulcer drug with HSP-inducing activity, suppressed ethanol-induced gastric lesions in wild-type mice but not in heat shock factor 1 (HSF1)-null mice. The results suggest that the aggravation of irritant-induced gastric lesions in HSF1-null mice is due to their inability to up-regulate HSPs, leading to apoptosis. It is also suggested that the HSP-inducing activity of GGA contributes to the drug's antiulcer activity. This study provides direct genetic evidence that HSPs, after their HSF1-dependent up-regulation, confer gastric protection against the irritant-induced lesions.

AB - Gastric lesions result from an imbalance between aggressive and defensive factors. Indirect lines of evidence suggest that heat shock proteins (HSPs) induced by various aggressive factors provide a major protective mechanism. In this study, we compared gastric ulcerogenic response in wild-type mice and in those lacking heat shock factor 1 (HSF1), a transcription factor for hsp genes. The severity of gastric lesions induced by ethanol or hydrochloric acid was worsened in HSF1-null mice. Immunoblotting, real-time reverse transcription-polymerase chain reaction, immunohistochemical analysis, and terminal deoxynucleotidyl transferase dUTP nick-end labeling assay revealed that the ethanol administration up-regulated gastric mucosal HSPs, in particular HSP70, in an HSF1-dependent manner, and more apoptotic cells were observed in the gastric mucosa of HSF1-null mice than in wild-type mice. In contrast, other parameters governing the gastric ulcerogenic response, including gastric acid secretion, gastric mucosal blood flow, and prostaglandin E2 levels, were not significantly affected by the absence of the hsf1 gene. Geranylgeranylacetone (GGA), a clinically used antiulcer drug with HSP-inducing activity, suppressed ethanol-induced gastric lesions in wild-type mice but not in heat shock factor 1 (HSF1)-null mice. The results suggest that the aggravation of irritant-induced gastric lesions in HSF1-null mice is due to their inability to up-regulate HSPs, leading to apoptosis. It is also suggested that the HSP-inducing activity of GGA contributes to the drug's antiulcer activity. This study provides direct genetic evidence that HSPs, after their HSF1-dependent up-regulation, confer gastric protection against the irritant-induced lesions.

UR - http://www.scopus.com/inward/record.url?scp=33947367704&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947367704&partnerID=8YFLogxK

U2 - 10.1124/mol.106.033282

DO - 10.1124/mol.106.033282

M3 - Article

VL - 71

SP - 985

EP - 993

JO - Molecular Pharmacology

JF - Molecular Pharmacology

SN - 0026-895X

IS - 4

ER -