Genetic fate mapping of Olig2 progenitors in the injured adult cerebral cortex reveals preferential differentiation into astrocytes

Kouko Tatsumi, Hirohide Takebayashi, Takayuki Manabe, Kenji Tanaka, Manabu Makinodan, Takahira Yamauchi, Eri Makinodan, Hiroko Matsuyoshi, Hiroaki Okuda, Kazuhiro Ikenaka, Akio Wanaka

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Olig2 is a basic helix-loop-helix (bHLH) transcription factor essential for development of motoneurons and oligodendrocytes. It is known that Olig2+ cells persist in the central nervous system (CNS) from embryonic to adult stages and that the number of Olig2+ progenitors increases in the injured adult CNS. Recent studies have demonstrated an inhibitory action of Olig2 on neurogenesis in adult CNS, but the fate of Olig2+ cells in the injured state remains largely unknown. To trace directly the fate of Olig2 cells in the adult cerebral cortex after injury, we employed the CreER/loxP system to target the olig2 locus. In this genetic tracing study, green fluorescent protein (GFP) reporter-positive cells labeled after cryoinjury coexpressed glial fibrillary acidic protein (GFAP), an astrocytic marker. Electron microscopy also showed that GFP+ cells have the ultrastructural characteristics of astrocytes. Furthermore, GFP+ cells labeled before injury, most of which had been NG2 cells, also produced bushy astrocytes. Here we show direct evidence that Olig2+ cells preferentially differentiate into astrocytes, which strongly express GFAP, in response to injury in the adult cerebral cortex. These results suggest that reactive astrocytes, known to be the main contributors to glial scars, originate, at least in part, from Olig2+ cells.

Original languageEnglish
Pages (from-to)3494-3502
Number of pages9
JournalJournal of Neuroscience Research
Volume86
Issue number16
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Astrocytes
Cerebral Cortex
Green Fluorescent Proteins
Central Nervous System
Glial Fibrillary Acidic Protein
Wounds and Injuries
Basic Helix-Loop-Helix Transcription Factors
Neurogenesis
Oligodendroglia
Motor Neurons
Neuroglia
Cicatrix
Electron Microscopy

Keywords

  • Adult cerebral cortex
  • Astrocyte
  • CreER/loxP system
  • Olig2
  • Progenitor cells

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Genetic fate mapping of Olig2 progenitors in the injured adult cerebral cortex reveals preferential differentiation into astrocytes. / Tatsumi, Kouko; Takebayashi, Hirohide; Manabe, Takayuki; Tanaka, Kenji; Makinodan, Manabu; Yamauchi, Takahira; Makinodan, Eri; Matsuyoshi, Hiroko; Okuda, Hiroaki; Ikenaka, Kazuhiro; Wanaka, Akio.

In: Journal of Neuroscience Research, Vol. 86, No. 16, 2008, p. 3494-3502.

Research output: Contribution to journalArticle

Tatsumi, K, Takebayashi, H, Manabe, T, Tanaka, K, Makinodan, M, Yamauchi, T, Makinodan, E, Matsuyoshi, H, Okuda, H, Ikenaka, K & Wanaka, A 2008, 'Genetic fate mapping of Olig2 progenitors in the injured adult cerebral cortex reveals preferential differentiation into astrocytes', Journal of Neuroscience Research, vol. 86, no. 16, pp. 3494-3502. https://doi.org/10.1002/jnr.21862
Tatsumi, Kouko ; Takebayashi, Hirohide ; Manabe, Takayuki ; Tanaka, Kenji ; Makinodan, Manabu ; Yamauchi, Takahira ; Makinodan, Eri ; Matsuyoshi, Hiroko ; Okuda, Hiroaki ; Ikenaka, Kazuhiro ; Wanaka, Akio. / Genetic fate mapping of Olig2 progenitors in the injured adult cerebral cortex reveals preferential differentiation into astrocytes. In: Journal of Neuroscience Research. 2008 ; Vol. 86, No. 16. pp. 3494-3502.
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