Genetic mapping of 40 cDNA clones on the mouse genome by PCR

Minoru Ko, X. Wang, J. H. Horton, M. D. Hagen, N. Takahashi, Y. Maezaki, J. H. Nadeau

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We recently proposed a new PCR-based genetic marker assay for the mouse genome that exploits sequence differences in the 3′-untranslated region (UTR) of cDNAs between different mouse strains, called "biallelic polymorphic expressed sequence tags (bESTs)." The specific use of 3′-UTR has several advantages: (1) frequent sequence polymorphism between different mouse strains, (2) most commonly uninterrupted by introns, (3) usually unique sequence even among closely related gene family members. In this paper, we identify additional genetic loci defined by bEST and determine their location on the mouse genetic map by using interspecific backross mapping panels between C57BL/6J and Mus spretus. Of 136 markers tested, 86 produced unique PCR products from C57BL/6J and M. spretus genomic DNAs. We then sequenced these 86 PCR products from C57BL/6J and M. spretus and found that 59 markers have sequence polymorphisms. Of these, we mapped 36 by restriction fragment length polymorphism (RFLP) of the PCR products and 4 by length polymorphism (LP) of the PCR products. We discuss the possibility of a large-scale application of this method for cDNA mapping.

Original languageEnglish
Pages (from-to)349-355
Number of pages7
JournalMammalian Genome
Volume5
Issue number6
DOIs
Publication statusPublished - 1994 Jun
Externally publishedYes

Fingerprint

Complementary DNA
Clone Cells
Genome
Polymerase Chain Reaction
Expressed Sequence Tags
3' Untranslated Regions
Genetic Loci
Genetic Markers
Restriction Fragment Length Polymorphisms
Introns
DNA
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Ko, M., Wang, X., Horton, J. H., Hagen, M. D., Takahashi, N., Maezaki, Y., & Nadeau, J. H. (1994). Genetic mapping of 40 cDNA clones on the mouse genome by PCR. Mammalian Genome, 5(6), 349-355. https://doi.org/10.1007/BF00356553

Genetic mapping of 40 cDNA clones on the mouse genome by PCR. / Ko, Minoru; Wang, X.; Horton, J. H.; Hagen, M. D.; Takahashi, N.; Maezaki, Y.; Nadeau, J. H.

In: Mammalian Genome, Vol. 5, No. 6, 06.1994, p. 349-355.

Research output: Contribution to journalArticle

Ko, M, Wang, X, Horton, JH, Hagen, MD, Takahashi, N, Maezaki, Y & Nadeau, JH 1994, 'Genetic mapping of 40 cDNA clones on the mouse genome by PCR', Mammalian Genome, vol. 5, no. 6, pp. 349-355. https://doi.org/10.1007/BF00356553
Ko M, Wang X, Horton JH, Hagen MD, Takahashi N, Maezaki Y et al. Genetic mapping of 40 cDNA clones on the mouse genome by PCR. Mammalian Genome. 1994 Jun;5(6):349-355. https://doi.org/10.1007/BF00356553
Ko, Minoru ; Wang, X. ; Horton, J. H. ; Hagen, M. D. ; Takahashi, N. ; Maezaki, Y. ; Nadeau, J. H. / Genetic mapping of 40 cDNA clones on the mouse genome by PCR. In: Mammalian Genome. 1994 ; Vol. 5, No. 6. pp. 349-355.
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