Genetic mutation analysis of the malignant transformation of sinonasal inverted papilloma by targeted amplicon sequencing

Shinichiro Yasukawa, Satoshi Kano, Hiromitsu Hatakeyama, Yuji Nakamaru, Dai Takagi, Takatsugu Mizumachi, Masanobu Suzuki, Takayoshi Suzuki, Akira Nakazono, Shinya Tanaka, Hiroshi Nishihara, Akihiro Homma

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Background: The mechanism underlying the malignant transformation of inverted papilloma (IP) has not yet been elucidated. Methods: To clarify the genes responsible for the malignant transformation, we analyzed 10 cases of IP, 8 of IP with dysplasia, and 11 of squamous cell carcinoma (SCC) by targeted amplicon sequencing. Results: The number of mutant genes increased in the order of IP < dysplasia < SCC. Significant differences were observed in the mutation rates of three genes (KRAS, APC and STK11) in particular. TP53 was altered frequently in each group and might be involved in malignant transformation based on to the site of the mutation. A comparison of the genetic variants by region of IP tissue among patients with IP alone, and those with dysplasia or SCC revealed significant differences in the mutation rate of the KRAS gene. Conclusion: Identification of genetic mutations in KRAS is effective for predicting the malignant transformation of IP.

Original languageEnglish
Pages (from-to)835-843
Number of pages9
JournalInternational Journal of Clinical Oncology
Volume23
Issue number5
DOIs
Publication statusPublished - 2018 Oct 1

Keywords

  • Inverted papilloma
  • Malignant transformation
  • NGS
  • Squamous cell carcinoma
  • Targeted amplicon sequence

ASJC Scopus subject areas

  • Surgery
  • Hematology
  • Oncology

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