Genetic transfer of large DNA inserts to designated Loci of the Bacillus subtilis 168 genome

Mitsuhiro Itaya

Research output: Contribution to journalArticle

17 Citations (Scopus)


It was found that contiguous DNA segments of up to 50 kb can be transferred between Bacillus subtilis genomes when a sufficient length of the flanking genomic region is provided for homologous recombination, although the efficiency of transfer was reduced as the insert size increased. Inserts were translocated to different loci, where appropriate integration sites were created.

Original languageEnglish
Pages (from-to)1045-1048
Number of pages4
JournalJournal of Bacteriology
Issue number3
Publication statusPublished - 1999 Feb
Externally publishedYes


ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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