Genome-wide analysis of gene expression in synovial sarcomas using a cDNA microarray

Satoshi Nagayama, Toyomasa Katagiri, Tatsuhiko Tsunoda, Taisuke Hosaka, Yasuaki Nakashima, Nobuhito Araki, Katsuyuki Kusuzaki, Tomitaka Nakayama, Tadao Tsuboyama, Takashi Nakamura, Masayuki Imamura, Yusuke Nakamura, Junya Toguchida

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Among a histologically heterogeneous group of soft tissue sarcomas, synovial sarcoma (SS) is regarded as a "miscellaneous" entity of uncertain origin. Although recent molecular analysis has disclosed involvement of a specific chromosomal translocation in the pathogenesis of SS, its genetic features remain largely unclear. In the work reported here we examined genome-wide gene expression profiles of 13 SS cases and 34 other spindle-cell sarcoma cases by cDNA microarray consisting of 23,040 genes. A hierarchical clustering analysis grouped SS and malignant peripheral nerve sheath tumor into the same category, and these two types of tumor shared expression patterns of numerous genes relating to neural differentiation. Several genes were up-regulated in almost all SS cases, and the presumed functions of known genes among them were related to migration or differentiation of neural crest cells, suggesting the possibility of neuroectodermal origin of SS. Moreover, we identified a set of genes that divided SS cases into two putative subclasses, a feature that may shed light on novel biological aspects of SS in addition to those having to do with epithelial differentiation. These data have provided clues for understanding the origin and tumorigenesis of SS.

Original languageEnglish
Pages (from-to)5859-5866
Number of pages8
JournalCancer Research
Volume62
Issue number20
Publication statusPublished - 2002 Oct 15
Externally publishedYes

Fingerprint

Synovial Sarcoma
Oligonucleotide Array Sequence Analysis
Genome
Gene Expression
Genes
Sarcoma
Genetic Translocation
Neural Crest
Neurilemmoma
Transcriptome
Cluster Analysis
Carcinogenesis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nagayama, S., Katagiri, T., Tsunoda, T., Hosaka, T., Nakashima, Y., Araki, N., ... Toguchida, J. (2002). Genome-wide analysis of gene expression in synovial sarcomas using a cDNA microarray. Cancer Research, 62(20), 5859-5866.

Genome-wide analysis of gene expression in synovial sarcomas using a cDNA microarray. / Nagayama, Satoshi; Katagiri, Toyomasa; Tsunoda, Tatsuhiko; Hosaka, Taisuke; Nakashima, Yasuaki; Araki, Nobuhito; Kusuzaki, Katsuyuki; Nakayama, Tomitaka; Tsuboyama, Tadao; Nakamura, Takashi; Imamura, Masayuki; Nakamura, Yusuke; Toguchida, Junya.

In: Cancer Research, Vol. 62, No. 20, 15.10.2002, p. 5859-5866.

Research output: Contribution to journalArticle

Nagayama, S, Katagiri, T, Tsunoda, T, Hosaka, T, Nakashima, Y, Araki, N, Kusuzaki, K, Nakayama, T, Tsuboyama, T, Nakamura, T, Imamura, M, Nakamura, Y & Toguchida, J 2002, 'Genome-wide analysis of gene expression in synovial sarcomas using a cDNA microarray', Cancer Research, vol. 62, no. 20, pp. 5859-5866.
Nagayama S, Katagiri T, Tsunoda T, Hosaka T, Nakashima Y, Araki N et al. Genome-wide analysis of gene expression in synovial sarcomas using a cDNA microarray. Cancer Research. 2002 Oct 15;62(20):5859-5866.
Nagayama, Satoshi ; Katagiri, Toyomasa ; Tsunoda, Tatsuhiko ; Hosaka, Taisuke ; Nakashima, Yasuaki ; Araki, Nobuhito ; Kusuzaki, Katsuyuki ; Nakayama, Tomitaka ; Tsuboyama, Tadao ; Nakamura, Takashi ; Imamura, Masayuki ; Nakamura, Yusuke ; Toguchida, Junya. / Genome-wide analysis of gene expression in synovial sarcomas using a cDNA microarray. In: Cancer Research. 2002 ; Vol. 62, No. 20. pp. 5859-5866.
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