Genome-wide distribution of linker histone H1.0 is independent of MeCP2

Aya Ito-Ishida, Hari Krishna Yamalanchili, Yingyao Shao, Steven A. Baker, Laura D. Heckman, Laura A. Lavery, Ji Yoen Kim, Laura M. Lombardi, Yaling Sun, Zhandong Liu, Huda Y. Zoghbi

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3 Citations (Scopus)

Abstract

Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent.

Original languageEnglish
Pages (from-to)794-798
Number of pages5
JournalNature Neuroscience
Volume21
Issue number6
DOIs
Publication statusPublished - 2018 Jun 1

ASJC Scopus subject areas

  • Neuroscience(all)

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    Ito-Ishida, A., Yamalanchili, H. K., Shao, Y., Baker, S. A., Heckman, L. D., Lavery, L. A., Kim, J. Y., Lombardi, L. M., Sun, Y., Liu, Z., & Zoghbi, H. Y. (2018). Genome-wide distribution of linker histone H1.0 is independent of MeCP2. Nature Neuroscience, 21(6), 794-798. https://doi.org/10.1038/s41593-018-0155-8