Genome-wide DNA methylation profiles in renal tumors of various histological subtypes and non-tumorous renal tissues

Eri Arai, Saori Wakai-Ushijima, Hiroyuki Fujimoto, Fumie Hosoda, Tatsuhiro Shibata, Tadashi Kondo, Sana Yokoi, Issei Imoto, Johji Inazawa, Setsuo Hirohashi, Yae Kanai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: The aim of this study is to clarify genome-wide DNA methylation profiles in renal tumors of various histological subtypes. Methods: Bacterial artificial chromosome (BAC) array-based methylated CpG island amplification was performed using tissue samples of 17 patients with papillary renal cell carcinomas (RCCs), chromophobe RCCs and oncocytomas, and the results were compared with those from 51 patients with clear cell RCCs. Results: Unsupervised hierarchical clustering analysis based on DNA methylation status clustered type 1 and type 2 papillary RCCs into different subclasses. Although chromophobe RCCs and oncocytomas were clustered into the same subclass, the DNA methylation status of 21 BAC clones was able to discriminate chromophobe RCCs from oncocytomas. The number of BAC clones showing DNA methylation alteration in non-tumorous renal tissue from patients with chromophobe RCCs and oncocytomas was smaller than that from patients with clear cell RCCs. Biphasic accumulation of DNA methylation alterations was observed in non-tumorous renal tissue from all 68 patients, and patients showing such alterations on more BAC clones had a poorer outcome than patients showing them on fewer BAC clones. Conclusions: DNA methylation profiles determining the histological subtypes of renal tumors developing in individual patients and/or patient outcome may be already established in non-tumorous renal tissue at the precancerous stage.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalPathobiology
Volume78
Issue number1
DOIs
Publication statusPublished - 2011 Apr
Externally publishedYes

Fingerprint

DNA Methylation
Renal Cell Carcinoma
Bacterial Artificial Chromosomes
Genome
Kidney
Neoplasms
Clone Cells
Oxyphilic Adenoma
CpG Islands
Cluster Analysis

Keywords

  • Chromophobe renal cell carcinoma
  • Clear cell renal cell carcinoma
  • DNA methylation
  • Oncocytoma
  • Papillary renal cell carcinoma
  • Precancerous condition

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Genome-wide DNA methylation profiles in renal tumors of various histological subtypes and non-tumorous renal tissues. / Arai, Eri; Wakai-Ushijima, Saori; Fujimoto, Hiroyuki; Hosoda, Fumie; Shibata, Tatsuhiro; Kondo, Tadashi; Yokoi, Sana; Imoto, Issei; Inazawa, Johji; Hirohashi, Setsuo; Kanai, Yae.

In: Pathobiology, Vol. 78, No. 1, 04.2011, p. 1-9.

Research output: Contribution to journalArticle

Arai, E, Wakai-Ushijima, S, Fujimoto, H, Hosoda, F, Shibata, T, Kondo, T, Yokoi, S, Imoto, I, Inazawa, J, Hirohashi, S & Kanai, Y 2011, 'Genome-wide DNA methylation profiles in renal tumors of various histological subtypes and non-tumorous renal tissues', Pathobiology, vol. 78, no. 1, pp. 1-9. https://doi.org/10.1159/000322072
Arai, Eri ; Wakai-Ushijima, Saori ; Fujimoto, Hiroyuki ; Hosoda, Fumie ; Shibata, Tatsuhiro ; Kondo, Tadashi ; Yokoi, Sana ; Imoto, Issei ; Inazawa, Johji ; Hirohashi, Setsuo ; Kanai, Yae. / Genome-wide DNA methylation profiles in renal tumors of various histological subtypes and non-tumorous renal tissues. In: Pathobiology. 2011 ; Vol. 78, No. 1. pp. 1-9.
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