Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome

Minoru Ko, Tracy A. Threat, Xueqian Wang, Joseph H. Horton, Yushun Cui, Xiaohong Wang, Eric Pryor, Jason Paris, Jeannine Wells-Smith, John R. Kitchen, Lucy B. Rowe, Janan Eppig, Toshihiko Satoh, Larry Brant, Hiroyuki Fujiwara, Shinichi Yotsumoto, Hiroshi Nakashima

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Mammalian embryos can only survive if they attach to the uterus (implantation) and establish proper maternal-fetal interactions. To understand this complex implantation pathway, we have initiated genomic analysis with a systematic study of the cohort of genes expressed in extraembryonic cells that are derived from the conceptus and play a major role in this process. A total of 2103 cDNAs from the extraembryonic portion of 7.5-day post-conception mouse embryos yielded 3186 expressed sequence tags, ~40% of which were novel to the sequence databases. Furthermore, when 155 of the cDNA clones with no homology to previously detected genes were genetically mapped, apparent clustering of these expressed genes was detected in subregions of chromosomes 2, 7, 9 and 17, with 6.5% of the observed genes localized in the t-complex region of chromosome 17, which represents only ~1.5% of the mouse genome. In contrast, X-linked genes were under-represented. Semi-quantitative RT-PCR analyses of the mapped genes demonstrated that one third of the genes were expressed solely in extraembryonic tissue and an additional one third of the genes were expressed predominantly in the extraembryonic tissues. The over-representation of extraembryonic-expressed genes in dosage-sensitive autosomal imprinted regions and under-representation on the dosage-compensated X chromosome may reflect a need for tight quantitative control of expression during development.

Original languageEnglish
Pages (from-to)1967-1978
Number of pages12
JournalHuman Molecular Genetics
Volume7
Issue number12
DOIs
Publication statusPublished - 1998 Nov
Externally publishedYes

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Chromosome Mapping
X Chromosome
Embryonic Structures
Genes
Chromosomes, Human, Pair 17
t-Complex Genome Region
Complementary DNA
Mammalian Embryo
X-Linked Genes
Chromosomes, Human, Pair 7
Chromosomes, Human, Pair 2
Gene Dosage
Expressed Sequence Tags
Uterus
Cluster Analysis
Cohort Studies
Clone Cells
Mothers
Genome
Databases

ASJC Scopus subject areas

  • Genetics

Cite this

Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome. / Ko, Minoru; Threat, Tracy A.; Wang, Xueqian; Horton, Joseph H.; Cui, Yushun; Wang, Xiaohong; Pryor, Eric; Paris, Jason; Wells-Smith, Jeannine; Kitchen, John R.; Rowe, Lucy B.; Eppig, Janan; Satoh, Toshihiko; Brant, Larry; Fujiwara, Hiroyuki; Yotsumoto, Shinichi; Nakashima, Hiroshi.

In: Human Molecular Genetics, Vol. 7, No. 12, 11.1998, p. 1967-1978.

Research output: Contribution to journalArticle

Ko, M, Threat, TA, Wang, X, Horton, JH, Cui, Y, Wang, X, Pryor, E, Paris, J, Wells-Smith, J, Kitchen, JR, Rowe, LB, Eppig, J, Satoh, T, Brant, L, Fujiwara, H, Yotsumoto, S & Nakashima, H 1998, 'Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome', Human Molecular Genetics, vol. 7, no. 12, pp. 1967-1978. https://doi.org/10.1093/hmg/7.12.1967
Ko, Minoru ; Threat, Tracy A. ; Wang, Xueqian ; Horton, Joseph H. ; Cui, Yushun ; Wang, Xiaohong ; Pryor, Eric ; Paris, Jason ; Wells-Smith, Jeannine ; Kitchen, John R. ; Rowe, Lucy B. ; Eppig, Janan ; Satoh, Toshihiko ; Brant, Larry ; Fujiwara, Hiroyuki ; Yotsumoto, Shinichi ; Nakashima, Hiroshi. / Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome. In: Human Molecular Genetics. 1998 ; Vol. 7, No. 12. pp. 1967-1978.
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abstract = "Mammalian embryos can only survive if they attach to the uterus (implantation) and establish proper maternal-fetal interactions. To understand this complex implantation pathway, we have initiated genomic analysis with a systematic study of the cohort of genes expressed in extraembryonic cells that are derived from the conceptus and play a major role in this process. A total of 2103 cDNAs from the extraembryonic portion of 7.5-day post-conception mouse embryos yielded 3186 expressed sequence tags, ~40{\%} of which were novel to the sequence databases. Furthermore, when 155 of the cDNA clones with no homology to previously detected genes were genetically mapped, apparent clustering of these expressed genes was detected in subregions of chromosomes 2, 7, 9 and 17, with 6.5{\%} of the observed genes localized in the t-complex region of chromosome 17, which represents only ~1.5{\%} of the mouse genome. In contrast, X-linked genes were under-represented. Semi-quantitative RT-PCR analyses of the mapped genes demonstrated that one third of the genes were expressed solely in extraembryonic tissue and an additional one third of the genes were expressed predominantly in the extraembryonic tissues. The over-representation of extraembryonic-expressed genes in dosage-sensitive autosomal imprinted regions and under-representation on the dosage-compensated X chromosome may reflect a need for tight quantitative control of expression during development.",
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AU - Eppig, Janan

AU - Satoh, Toshihiko

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