Genomic approaches to early embryogenesis and stem cell biology

Kazuhiro Aiba, Mark G. Carter, Ryo Matoba, Minoru S.H. Ko

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

Large-scale systematic gene expression analyses of early embryos and stem cells provide useful information to identify genes expressed differentially or uniquely in these cells. We review the current status of various approaches applied to preimplantation embryos and stem cells: expressed sequence tag, serial analysis of gene expression, differential display, massively parallel signature sequencing, DNA microarray (DNA chip) analysis, and chromatin-immunoprecipitation microarrays. We also discuss the biological questions that can only be addressed by the analysis of global gene expression patterns, such as so-called stemness and developmental potency. As the emphasis now shifts from expression profiling to functional studies, we review the genome-scale functional studies of genes: expression cloning, gene trapping, RNA interference, and gene disruptions. Finally, we discuss the future clinical application of such methodologies.

Original languageEnglish
Pages (from-to)330-339
Number of pages10
JournalSeminars in Reproductive Medicine
Volume24
Issue number5
DOIs
Publication statusPublished - 2006 Nov 1
Externally publishedYes

Keywords

  • Embryogenomics
  • Large-scale analysis
  • Microarray
  • Preimplantation embryos
  • Stem cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Endocrinology
  • Obstetrics and Gynaecology
  • Physiology (medical)

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