Genomic expressions of human T-lymphotropic virus (HTLV-I).

M. Hatanaka, H. Sabe, A. Tanaka, K. Mori, H. Siomi, S. Nam, Y. Adachi, S. Itamura, K. Hirayoshi

Research output: Contribution to journalArticle

Abstract

Human T-lymphocyte cell line termed MT-2 is producing persistently HTLV-I virion and has a strong potential to transform human T-lymphocytes when cocultivated. The virion of HTLV-I (MT-2) was isolated and its RNA was extracted to analyze the gene and gene products of HTLV-I. HTLV (MT-2) virion RNA was translated in a rabbit reticulocyte lysate system in vitro in which a gag precursor polyprotein (p53) and a putative gag-prt fusion protein (p76) were synthesized from a full length 35S RNA. The full length provirus, HTLV-I (MT-2), was molecularly cloned and its genomic expression was examined transiently and permanently by transfecting in human lymphoid and non-lymphoid cells. The cloned provirus expressed the same virological activities as observed in naturally occurring infection of the virus. A new protease gene of HTLV-I was found and its function of the gene product was studied.

Original languageEnglish
Pages (from-to)S79-85
JournalAIDS research
Volume2 Suppl 1
Publication statusPublished - 1986 Dec
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Virology

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    Hatanaka, M., Sabe, H., Tanaka, A., Mori, K., Siomi, H., Nam, S., Adachi, Y., Itamura, S., & Hirayoshi, K. (1986). Genomic expressions of human T-lymphotropic virus (HTLV-I). AIDS research, 2 Suppl 1, S79-85.