Genomic expressions of human T-lymphotropic virus (HTLV-I).

M. Hatanaka, H. Sabe, A. Tanaka, K. Mori, Haruhiko Siomi, S. Nam, Y. Adachi, S. Itamura, K. Hirayoshi

Research output: Contribution to journalArticle

Abstract

Human T-lymphocyte cell line termed MT-2 is producing persistently HTLV-I virion and has a strong potential to transform human T-lymphocytes when cocultivated. The virion of HTLV-I (MT-2) was isolated and its RNA was extracted to analyze the gene and gene products of HTLV-I. HTLV (MT-2) virion RNA was translated in a rabbit reticulocyte lysate system in vitro in which a gag precursor polyprotein (p53) and a putative gag-prt fusion protein (p76) were synthesized from a full length 35S RNA. The full length provirus, HTLV-I (MT-2), was molecularly cloned and its genomic expression was examined transiently and permanently by transfecting in human lymphoid and non-lymphoid cells. The cloned provirus expressed the same virological activities as observed in naturally occurring infection of the virus. A new protease gene of HTLV-I was found and its function of the gene product was studied.

Original languageEnglish
JournalAIDS Research
Volume2 Suppl 1
Publication statusPublished - 1986 Dec
Externally publishedYes

Fingerprint

Human T-lymphotropic virus 2
Viruses
Virion
Proviruses
RNA
Genes
T-Lymphocytes
gag Gene Products
Human T-lymphotropic virus 1
Reticulocytes
Virus Diseases
Peptide Hydrolases
Rabbits
Cell Line
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Hatanaka, M., Sabe, H., Tanaka, A., Mori, K., Siomi, H., Nam, S., ... Hirayoshi, K. (1986). Genomic expressions of human T-lymphotropic virus (HTLV-I). AIDS Research, 2 Suppl 1.

Genomic expressions of human T-lymphotropic virus (HTLV-I). / Hatanaka, M.; Sabe, H.; Tanaka, A.; Mori, K.; Siomi, Haruhiko; Nam, S.; Adachi, Y.; Itamura, S.; Hirayoshi, K.

In: AIDS Research, Vol. 2 Suppl 1, 12.1986.

Research output: Contribution to journalArticle

Hatanaka, M, Sabe, H, Tanaka, A, Mori, K, Siomi, H, Nam, S, Adachi, Y, Itamura, S & Hirayoshi, K 1986, 'Genomic expressions of human T-lymphotropic virus (HTLV-I).', AIDS Research, vol. 2 Suppl 1.
Hatanaka M, Sabe H, Tanaka A, Mori K, Siomi H, Nam S et al. Genomic expressions of human T-lymphotropic virus (HTLV-I). AIDS Research. 1986 Dec;2 Suppl 1.
Hatanaka, M. ; Sabe, H. ; Tanaka, A. ; Mori, K. ; Siomi, Haruhiko ; Nam, S. ; Adachi, Y. ; Itamura, S. ; Hirayoshi, K. / Genomic expressions of human T-lymphotropic virus (HTLV-I). In: AIDS Research. 1986 ; Vol. 2 Suppl 1.
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