Genomic sequences encoding two types of medaka hemopexin-like protein Wap65, and their gene expression profiles in embryos

Makiko Nakaniwa, Makoto Hirayama, Atsushi Shimizu, Takashi Sasaki, Shuichi Asakawa, Nobuyoshi Shimizu, Shugo Watabe

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Medaka genomic BAC clones, which contained two types of medaka hemopexin-like protein gene (Wap65), mWap65-1 and mWap65-2, were screened and their genomic sequences were determined by the shotgun strategy. The exon-intron organizations were highly conserved between both mWap65s and human hemopexin genes. The 5′-flanking regions of mWap65-1 and mWap65-2 contained various putative transcription factor binding sites including elements for developmental regulation. The expression patterns of mWap65s during embryonic development were examined by quantitative real-time PCR, demonstrating that both mWap65 transcripts were observed in early embryonic stages, but their expression patterns were different. Interestingly, in situ hybridization revealed that mWap65-2 transcripts were restricted to liver, whereas mWap65-1 transcripts were detected along the edge of pectoral fin buds and the median fin fold of tail buds in embryos at stage 32. Furthermore, we generated transgenic medaka expressing GFP driven by mWap65-1 and mWap65-2 promoters and observed GFP expression patterns during ontogeny. Although localizations of GFP varied among individuals, embryos uniformly expressed GFP 1 day after injection of mWap65-1-hrGFP and mWap65-2-hrGFP constructs, suggesting that mWap65-1 and mWap65-2 promoters were activated in very early stages. The differences between mWap65-1 and mWap65-2 in their expression profiles indicate their distinct roles during ontogeny.

Original languageEnglish
Pages (from-to)1915-1925
Number of pages11
JournalJournal of Experimental Biology
Volume208
Issue number10
DOIs
Publication statusPublished - 2005 May 1

Keywords

  • Embryo
  • Gene expression
  • Genomic sequences
  • Hemopexin
  • Medaka
  • Oryzias latipes
  • Transgenic fish
  • mWap65-1
  • mWap65-2

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Aquatic Science
  • Animal Science and Zoology
  • Molecular Biology
  • Insect Science

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