Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes

Yuko Yao, Takehiro Yamamoto, Makiko Tsutsumi, Masaru Matsuda, Hiroshi Hori, Kiyoshi Naruse, Hiroshi Mitani, Akihiro Shima, Shuichi Asakawa, Nobuyoshi Shimizu, Norio Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A cDNA clone encoding the soluble guanylyl cyclase α2 subunit was isolated from medaka fish (Oryzias latipes) and designated as OIGCS-α2. The OIGCS-α2 cDNA was 3192 bp in length and the open reading frame (ORF) encodes a protein of 805 amino acids. The deduced amino acid sequence has high similarity to that of the mammalian α2 subunit gene except for the N-terminal regulatory domain. The C-terminal 5 amino acids, "RETSL", which have been reported to interact with the post synaptic density protein (PSD)-95 were conserved. An RNase protection assay with adult fish organs showed that OIGCS-α 2 was expressed mainly in the brain and testis. The complete nucleotide sequence (about 41 kbp) of the OIGCS-α2 genomic DNA clone isolated from a medaka fish BAC library indicated that the OIGCS-α2 gene consisted of 9 exons and 8 introns. The 5′-flanking region and larger introns, such as introns 1, 4, and 7, contained the several fragments conserved in the nucleotide sequences of Rex6 (non-long terminal repeat retrotransposon), MHC class I genomic region, and OIGC1, the medaka fish homolog of the mammalian guanylyl cyclase B gene. Linkage analysis on the medaka fish chromosome demonstrated that the OIGCS-α2 gene was mapped to LG13; this mapping position was different from those for the OIGCS-α2 and OIGCS-β 1 genes (LG1).

Original languageEnglish
Pages (from-to)1293-1304
Number of pages12
JournalZoological Science
Volume20
Issue number10
DOIs
Publication statusPublished - 2003 Oct
Externally publishedYes

Fingerprint

guanylate cyclase
Oryzias latipes
genomics
introns
fish
genes
clones
nucleotide sequences
amino acids
terminal repeat sequences
retrotransposons
exons
linkage (genetics)
open reading frames
testes
amino acid sequences
proteins
chromosomes
brain
DNA

Keywords

  • cGMP
  • Exon/intron organization
  • Gene expression
  • Medaka fish
  • Soluble guanylyl cyclase

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes. / Yao, Yuko; Yamamoto, Takehiro; Tsutsumi, Makiko; Matsuda, Masaru; Hori, Hiroshi; Naruse, Kiyoshi; Mitani, Hiroshi; Shima, Akihiro; Asakawa, Shuichi; Shimizu, Nobuyoshi; Suzuki, Norio.

In: Zoological Science, Vol. 20, No. 10, 10.2003, p. 1293-1304.

Research output: Contribution to journalArticle

Yao, Y, Yamamoto, T, Tsutsumi, M, Matsuda, M, Hori, H, Naruse, K, Mitani, H, Shima, A, Asakawa, S, Shimizu, N & Suzuki, N 2003, 'Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes', Zoological Science, vol. 20, no. 10, pp. 1293-1304. https://doi.org/10.2108/zsj.20.1293
Yao, Yuko ; Yamamoto, Takehiro ; Tsutsumi, Makiko ; Matsuda, Masaru ; Hori, Hiroshi ; Naruse, Kiyoshi ; Mitani, Hiroshi ; Shima, Akihiro ; Asakawa, Shuichi ; Shimizu, Nobuyoshi ; Suzuki, Norio. / Genomic Structure and Expression of the Soluble Guanylyl Cyclase α2 Subunit Gene in the Medaka Fish Oryzias latipes. In: Zoological Science. 2003 ; Vol. 20, No. 10. pp. 1293-1304.
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AU - Matsuda, Masaru

AU - Hori, Hiroshi

AU - Naruse, Kiyoshi

AU - Mitani, Hiroshi

AU - Shima, Akihiro

AU - Asakawa, Shuichi

AU - Shimizu, Nobuyoshi

AU - Suzuki, Norio

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