Genomic structure of the actin-encoding gene of Pneumocystis carinii

Yasushi Miyahira; Yoshiki Hiraoka; Naoki Komatsu; Tsutomu Takeuchi; Sadakazu Aiso

doi:10.1016/S1383-5769(97)00041-X

Genomic structure of the actin-encoding gene of Pneumocystis carinii

Yasushi Miyahira, Yoshiki Hiraoka, Naoki Komatsu, Tsutomu Takeuchi, Sadakazu Aiso

Department of Anatomy

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have isolated both the genomic and cDNA clones of the actin gene of Pneumocystis carinii and analyzed its genomic structure. The gene consists of eight exons. The introns are uniformly short in size (43-49 nucleotides). Multiple poly(A) sites of the actin mRNA are located in a highly AT-rich portion of the 3′-flanking region of this gene, although the canonical poly(A) signal, AATAAA, is not found in the region. Transcription initiation sites have been determined by the primer extension method. A TATA-box-like sequence is located 39-bp upstream of the 5′-most major initiation site.

Original language	English
Pages (from-to)	289-295
Number of pages	7
Journal	Parasitology International
Volume	46
Issue number	4
DOIs	https://doi.org/10.1016/S1383-5769(97)00041-X
Publication status	Published - 1997 Dec

Keywords

Actin
Genomic structure
Nucleotide sequence
Opportunistic infection
Pathogen
Pneumocystis carinii

ASJC Scopus subject areas

Parasitology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S1383-5769(97)00041-X

Cite this

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abstract = "We have isolated both the genomic and cDNA clones of the actin gene of Pneumocystis carinii and analyzed its genomic structure. The gene consists of eight exons. The introns are uniformly short in size (43-49 nucleotides). Multiple poly(A) sites of the actin mRNA are located in a highly AT-rich portion of the 3′-flanking region of this gene, although the canonical poly(A) signal, AATAAA, is not found in the region. Transcription initiation sites have been determined by the primer extension method. A TATA-box-like sequence is located 39-bp upstream of the 5′-most major initiation site.",

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T1 - Genomic structure of the actin-encoding gene of Pneumocystis carinii

AU - Miyahira, Yasushi

AU - Hiraoka, Yoshiki

AU - Komatsu, Naoki

AU - Takeuchi, Tsutomu

AU - Aiso, Sadakazu

PY - 1997/12

Y1 - 1997/12

N2 - We have isolated both the genomic and cDNA clones of the actin gene of Pneumocystis carinii and analyzed its genomic structure. The gene consists of eight exons. The introns are uniformly short in size (43-49 nucleotides). Multiple poly(A) sites of the actin mRNA are located in a highly AT-rich portion of the 3′-flanking region of this gene, although the canonical poly(A) signal, AATAAA, is not found in the region. Transcription initiation sites have been determined by the primer extension method. A TATA-box-like sequence is located 39-bp upstream of the 5′-most major initiation site.

AB - We have isolated both the genomic and cDNA clones of the actin gene of Pneumocystis carinii and analyzed its genomic structure. The gene consists of eight exons. The introns are uniformly short in size (43-49 nucleotides). Multiple poly(A) sites of the actin mRNA are located in a highly AT-rich portion of the 3′-flanking region of this gene, although the canonical poly(A) signal, AATAAA, is not found in the region. Transcription initiation sites have been determined by the primer extension method. A TATA-box-like sequence is located 39-bp upstream of the 5′-most major initiation site.

KW - Actin

KW - Genomic structure

KW - Nucleotide sequence

KW - Opportunistic infection

KW - Pathogen

KW - Pneumocystis carinii

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Genomic structure of the actin-encoding gene of Pneumocystis carinii

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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