Genomic structure of the sea lamprey growth hormone-encoding gene

Shunsuke Moriyama, Mayumi Oda, Akiyoshi Takahashi, Stacia A. Sower, Hiroshi Kawauchi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Growth hormone (GH) belongs to a family of pituitary hormones together with prolactin and somatolactin. In our previous study, GH and its cDNA were identified in the pituitary gland of the sea lamprey, Petromyzon marinus, an extant representative of the most ancient class of vertebrates, and isolated GH stimulated expression of insulin-like growth factor in the liver. The evidence suggests that GH is the ancestral hormone in the molecular evolution of the GH/PRL/SL family and that the endocrine mechanism for growth stimulation was established at an early stage in the evolution of vertebrates. To further understand the molecular evolution of the GH/PRL/SL gene family, we report the genomic structure of sea lamprey GH including its 5′-flanking region, being cloned by PCR using specific primers prepared from its cDNA. The sea lamprey GH gene consists of 13,604 bp, making it the largest of all the GH genes. The 5′-flanking region within 697 bp contains consensus sequences for a TATA box, two Pit-1/GHF-1, three TRE, and a CRE. The sea lamprey GH gene consists of five exons and four introns, the same as in mammals, birds, and teleosts such as cypriniforms and siluriforms with the exception of some teleosts such as salmoniforms, percififorms, and tetradontiforms, in which there is an additional intron in the 5th exon. The 5-exon-type gene organization might reflect the structure of the ancestral gene for the GH/PRL/SL gene family.

Original languageEnglish
Pages (from-to)33-40
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume148
Issue number1
DOIs
Publication statusPublished - 2006 Aug
Externally publishedYes

Fingerprint

Petromyzon
Petromyzon marinus
somatotropin
Growth Hormone
genomics
Genes
genes
exons
Exons
Molecular Evolution
5' Flanking Region
Introns
Vertebrates
introns
Complementary DNA
vertebrates
pituitary hormones
TATA box
TATA Box
Pituitary Hormones

Keywords

  • 5′-Flanking region
  • Exon-intron organization
  • Genomic structure
  • Growth hormone
  • Sea lamprey

ASJC Scopus subject areas

  • Endocrinology

Cite this

Genomic structure of the sea lamprey growth hormone-encoding gene. / Moriyama, Shunsuke; Oda, Mayumi; Takahashi, Akiyoshi; Sower, Stacia A.; Kawauchi, Hiroshi.

In: General and Comparative Endocrinology, Vol. 148, No. 1, 08.2006, p. 33-40.

Research output: Contribution to journalArticle

Moriyama, S, Oda, M, Takahashi, A, Sower, SA & Kawauchi, H 2006, 'Genomic structure of the sea lamprey growth hormone-encoding gene', General and Comparative Endocrinology, vol. 148, no. 1, pp. 33-40. https://doi.org/10.1016/j.ygcen.2005.09.023
Moriyama, Shunsuke ; Oda, Mayumi ; Takahashi, Akiyoshi ; Sower, Stacia A. ; Kawauchi, Hiroshi. / Genomic structure of the sea lamprey growth hormone-encoding gene. In: General and Comparative Endocrinology. 2006 ; Vol. 148, No. 1. pp. 33-40.
@article{5dbff55f93e34526b5617380ba94e389,
title = "Genomic structure of the sea lamprey growth hormone-encoding gene",
abstract = "Growth hormone (GH) belongs to a family of pituitary hormones together with prolactin and somatolactin. In our previous study, GH and its cDNA were identified in the pituitary gland of the sea lamprey, Petromyzon marinus, an extant representative of the most ancient class of vertebrates, and isolated GH stimulated expression of insulin-like growth factor in the liver. The evidence suggests that GH is the ancestral hormone in the molecular evolution of the GH/PRL/SL family and that the endocrine mechanism for growth stimulation was established at an early stage in the evolution of vertebrates. To further understand the molecular evolution of the GH/PRL/SL gene family, we report the genomic structure of sea lamprey GH including its 5′-flanking region, being cloned by PCR using specific primers prepared from its cDNA. The sea lamprey GH gene consists of 13,604 bp, making it the largest of all the GH genes. The 5′-flanking region within 697 bp contains consensus sequences for a TATA box, two Pit-1/GHF-1, three TRE, and a CRE. The sea lamprey GH gene consists of five exons and four introns, the same as in mammals, birds, and teleosts such as cypriniforms and siluriforms with the exception of some teleosts such as salmoniforms, percififorms, and tetradontiforms, in which there is an additional intron in the 5th exon. The 5-exon-type gene organization might reflect the structure of the ancestral gene for the GH/PRL/SL gene family.",
keywords = "5′-Flanking region, Exon-intron organization, Genomic structure, Growth hormone, Sea lamprey",
author = "Shunsuke Moriyama and Mayumi Oda and Akiyoshi Takahashi and Sower, {Stacia A.} and Hiroshi Kawauchi",
year = "2006",
month = "8",
doi = "10.1016/j.ygcen.2005.09.023",
language = "English",
volume = "148",
pages = "33--40",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Genomic structure of the sea lamprey growth hormone-encoding gene

AU - Moriyama, Shunsuke

AU - Oda, Mayumi

AU - Takahashi, Akiyoshi

AU - Sower, Stacia A.

AU - Kawauchi, Hiroshi

PY - 2006/8

Y1 - 2006/8

N2 - Growth hormone (GH) belongs to a family of pituitary hormones together with prolactin and somatolactin. In our previous study, GH and its cDNA were identified in the pituitary gland of the sea lamprey, Petromyzon marinus, an extant representative of the most ancient class of vertebrates, and isolated GH stimulated expression of insulin-like growth factor in the liver. The evidence suggests that GH is the ancestral hormone in the molecular evolution of the GH/PRL/SL family and that the endocrine mechanism for growth stimulation was established at an early stage in the evolution of vertebrates. To further understand the molecular evolution of the GH/PRL/SL gene family, we report the genomic structure of sea lamprey GH including its 5′-flanking region, being cloned by PCR using specific primers prepared from its cDNA. The sea lamprey GH gene consists of 13,604 bp, making it the largest of all the GH genes. The 5′-flanking region within 697 bp contains consensus sequences for a TATA box, two Pit-1/GHF-1, three TRE, and a CRE. The sea lamprey GH gene consists of five exons and four introns, the same as in mammals, birds, and teleosts such as cypriniforms and siluriforms with the exception of some teleosts such as salmoniforms, percififorms, and tetradontiforms, in which there is an additional intron in the 5th exon. The 5-exon-type gene organization might reflect the structure of the ancestral gene for the GH/PRL/SL gene family.

AB - Growth hormone (GH) belongs to a family of pituitary hormones together with prolactin and somatolactin. In our previous study, GH and its cDNA were identified in the pituitary gland of the sea lamprey, Petromyzon marinus, an extant representative of the most ancient class of vertebrates, and isolated GH stimulated expression of insulin-like growth factor in the liver. The evidence suggests that GH is the ancestral hormone in the molecular evolution of the GH/PRL/SL family and that the endocrine mechanism for growth stimulation was established at an early stage in the evolution of vertebrates. To further understand the molecular evolution of the GH/PRL/SL gene family, we report the genomic structure of sea lamprey GH including its 5′-flanking region, being cloned by PCR using specific primers prepared from its cDNA. The sea lamprey GH gene consists of 13,604 bp, making it the largest of all the GH genes. The 5′-flanking region within 697 bp contains consensus sequences for a TATA box, two Pit-1/GHF-1, three TRE, and a CRE. The sea lamprey GH gene consists of five exons and four introns, the same as in mammals, birds, and teleosts such as cypriniforms and siluriforms with the exception of some teleosts such as salmoniforms, percififorms, and tetradontiforms, in which there is an additional intron in the 5th exon. The 5-exon-type gene organization might reflect the structure of the ancestral gene for the GH/PRL/SL gene family.

KW - 5′-Flanking region

KW - Exon-intron organization

KW - Genomic structure

KW - Growth hormone

KW - Sea lamprey

UR - http://www.scopus.com/inward/record.url?scp=33745115305&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745115305&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2005.09.023

DO - 10.1016/j.ygcen.2005.09.023

M3 - Article

VL - 148

SP - 33

EP - 40

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 1

ER -