Genotype-phenotype linkage for directed evolution and screening of combinatorial protein libraries

Nobuhide Doi, H. Yanagawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The technologies for screening peptide and protein libraries for studies in the fields of directed protein evolution and functional genomics have advanced with astonishing speed. For screening of functional proteins, three technologies are required: (i) the construction of a gene library (genotype), (ii) the establishment of a linkage between each protein (phenotype) and its encoding gene (genotype), and (iii) the selection of desired proteins (phenotype) from the library. This review highlights the genotype-phenotype linkage technologies, which can be classified into three types; that is, cell-type linkage, virus-type linkage, and array-type linkage methods. These methods are summarized, and their advantages and disadvantages are discussed.

Original languageEnglish
Pages (from-to)497-509
Number of pages13
JournalCombinatorial Chemistry and High Throughput Screening
Volume4
Issue number6
Publication statusPublished - 2001

Fingerprint

Screening
Genotype
Proteins
Phenotype
Technology
Libraries
Peptide Library
Genomics
Viruses
Peptides

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Clinical Biochemistry
  • Pharmacology

Cite this

@article{bd00b3208ebd421191a845e1fc45b112,
title = "Genotype-phenotype linkage for directed evolution and screening of combinatorial protein libraries",
abstract = "The technologies for screening peptide and protein libraries for studies in the fields of directed protein evolution and functional genomics have advanced with astonishing speed. For screening of functional proteins, three technologies are required: (i) the construction of a gene library (genotype), (ii) the establishment of a linkage between each protein (phenotype) and its encoding gene (genotype), and (iii) the selection of desired proteins (phenotype) from the library. This review highlights the genotype-phenotype linkage technologies, which can be classified into three types; that is, cell-type linkage, virus-type linkage, and array-type linkage methods. These methods are summarized, and their advantages and disadvantages are discussed.",
author = "Nobuhide Doi and H. Yanagawa",
year = "2001",
language = "English",
volume = "4",
pages = "497--509",
journal = "Combinatorial Chemistry and High Throughput Screening",
issn = "1386-2073",
publisher = "Bentham Science Publishers B.V.",
number = "6",

}

TY - JOUR

T1 - Genotype-phenotype linkage for directed evolution and screening of combinatorial protein libraries

AU - Doi, Nobuhide

AU - Yanagawa, H.

PY - 2001

Y1 - 2001

N2 - The technologies for screening peptide and protein libraries for studies in the fields of directed protein evolution and functional genomics have advanced with astonishing speed. For screening of functional proteins, three technologies are required: (i) the construction of a gene library (genotype), (ii) the establishment of a linkage between each protein (phenotype) and its encoding gene (genotype), and (iii) the selection of desired proteins (phenotype) from the library. This review highlights the genotype-phenotype linkage technologies, which can be classified into three types; that is, cell-type linkage, virus-type linkage, and array-type linkage methods. These methods are summarized, and their advantages and disadvantages are discussed.

AB - The technologies for screening peptide and protein libraries for studies in the fields of directed protein evolution and functional genomics have advanced with astonishing speed. For screening of functional proteins, three technologies are required: (i) the construction of a gene library (genotype), (ii) the establishment of a linkage between each protein (phenotype) and its encoding gene (genotype), and (iii) the selection of desired proteins (phenotype) from the library. This review highlights the genotype-phenotype linkage technologies, which can be classified into three types; that is, cell-type linkage, virus-type linkage, and array-type linkage methods. These methods are summarized, and their advantages and disadvantages are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0034861907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034861907&partnerID=8YFLogxK

M3 - Article

C2 - 11562254

AN - SCOPUS:0034861907

VL - 4

SP - 497

EP - 509

JO - Combinatorial Chemistry and High Throughput Screening

JF - Combinatorial Chemistry and High Throughput Screening

SN - 1386-2073

IS - 6

ER -