Abstract
Microcystis aeruginosa is one of the bloom-forming harmful algae in freshwater ecosystems. We genetically characterized Microcystis populations during bloom-forming periods in various reservoirs, lakes, and ponds in Japan during 2009. Using phylogenetic analysis, we evaluated the relationship between current genotype expansions and geographic location within western Japan and intraspecific variation. Microcystis aeruginosa colonies were isolated at 15 sites and were analyzed by sequencing the 16S-23S internal transcribed spacer (ITS) region of the ribosomal operon, and the potential to produce toxins was assessed by PCR-based detection of the microcystin synthetase gene mcyG. In total, 171 colonies were separated into 41 genotypes. The highest genotypic composition was detected in the south basin of Lake Biwa and the lowest in Lagoon Iba. Cluster analysis indicated no obvious association between genotypic composition and geographic distance. Thus, clear genetic differentiation accompanied by geographic origins was not found in western Japan. The resulting neighbor-joining tree revealed 3 clusters, 2 of which contained strains that showed both nonamplification and amplification of the mcyG gene.
| Original language | English |
|---|---|
| Pages (from-to) | 266-272 |
| Number of pages | 7 |
| Journal | Canadian Journal of Microbiology |
| Volume | 59 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2013 Apr |
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Keywords
- Genotypic diversity
- Harmful algae
- ITS region
- Microcystis
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Microbiology
- Immunology
- Genetics
- Molecular Biology
Cite this
Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium microcystis aeruginosa in western Japan. / Ohbayashi, Kako; Hodoki, Yoshikuni; Kobayashi, Yuki; Okuda, Noboru; Nakano, Shin Ichi.
In: Canadian Journal of Microbiology, Vol. 59, No. 4, 04.2013, p. 266-272.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium microcystis aeruginosa in western Japan
AU - Ohbayashi, Kako
AU - Hodoki, Yoshikuni
AU - Kobayashi, Yuki
AU - Okuda, Noboru
AU - Nakano, Shin Ichi
PY - 2013/4
Y1 - 2013/4
N2 - Microcystis aeruginosa is one of the bloom-forming harmful algae in freshwater ecosystems. We genetically characterized Microcystis populations during bloom-forming periods in various reservoirs, lakes, and ponds in Japan during 2009. Using phylogenetic analysis, we evaluated the relationship between current genotype expansions and geographic location within western Japan and intraspecific variation. Microcystis aeruginosa colonies were isolated at 15 sites and were analyzed by sequencing the 16S-23S internal transcribed spacer (ITS) region of the ribosomal operon, and the potential to produce toxins was assessed by PCR-based detection of the microcystin synthetase gene mcyG. In total, 171 colonies were separated into 41 genotypes. The highest genotypic composition was detected in the south basin of Lake Biwa and the lowest in Lagoon Iba. Cluster analysis indicated no obvious association between genotypic composition and geographic distance. Thus, clear genetic differentiation accompanied by geographic origins was not found in western Japan. The resulting neighbor-joining tree revealed 3 clusters, 2 of which contained strains that showed both nonamplification and amplification of the mcyG gene.
AB - Microcystis aeruginosa is one of the bloom-forming harmful algae in freshwater ecosystems. We genetically characterized Microcystis populations during bloom-forming periods in various reservoirs, lakes, and ponds in Japan during 2009. Using phylogenetic analysis, we evaluated the relationship between current genotype expansions and geographic location within western Japan and intraspecific variation. Microcystis aeruginosa colonies were isolated at 15 sites and were analyzed by sequencing the 16S-23S internal transcribed spacer (ITS) region of the ribosomal operon, and the potential to produce toxins was assessed by PCR-based detection of the microcystin synthetase gene mcyG. In total, 171 colonies were separated into 41 genotypes. The highest genotypic composition was detected in the south basin of Lake Biwa and the lowest in Lagoon Iba. Cluster analysis indicated no obvious association between genotypic composition and geographic distance. Thus, clear genetic differentiation accompanied by geographic origins was not found in western Japan. The resulting neighbor-joining tree revealed 3 clusters, 2 of which contained strains that showed both nonamplification and amplification of the mcyG gene.
KW - Genotypic diversity
KW - Harmful algae
KW - ITS region
KW - Microcystis
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UR - http://www.scopus.com/inward/citedby.url?scp=84876232577&partnerID=8YFLogxK
U2 - 10.1139/cjm-2012-0724
DO - 10.1139/cjm-2012-0724
M3 - Article
C2 - 23586751
AN - SCOPUS:84876232577
VL - 59
SP - 266
EP - 272
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
SN - 0008-4166
IS - 4
ER -