Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium microcystis aeruginosa in western Japan

Kako Ohbayashi, Yoshikuni Hodoki, Yuki Kobayashi, Noboru Okuda, Shin Ichi Nakano

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Microcystis aeruginosa is one of the bloom-forming harmful algae in freshwater ecosystems. We genetically characterized Microcystis populations during bloom-forming periods in various reservoirs, lakes, and ponds in Japan during 2009. Using phylogenetic analysis, we evaluated the relationship between current genotype expansions and geographic location within western Japan and intraspecific variation. Microcystis aeruginosa colonies were isolated at 15 sites and were analyzed by sequencing the 16S-23S internal transcribed spacer (ITS) region of the ribosomal operon, and the potential to produce toxins was assessed by PCR-based detection of the microcystin synthetase gene mcyG. In total, 171 colonies were separated into 41 genotypes. The highest genotypic composition was detected in the south basin of Lake Biwa and the lowest in Lagoon Iba. Cluster analysis indicated no obvious association between genotypic composition and geographic distance. Thus, clear genetic differentiation accompanied by geographic origins was not found in western Japan. The resulting neighbor-joining tree revealed 3 clusters, 2 of which contained strains that showed both nonamplification and amplification of the mcyG gene.

Original languageEnglish
Pages (from-to)266-272
Number of pages7
JournalCanadian Journal of Microbiology
Volume59
Issue number4
DOIs
Publication statusPublished - 2013 Apr

Fingerprint

Microcystis
Cyanobacteria
Japan
Lakes
Genotype
Geographic Locations
Gene Amplification
Operon
Ligases
Fresh Water
Ecosystem
Cluster Analysis
Polymerase Chain Reaction
Population
Genes

Keywords

  • Genotypic diversity
  • Harmful algae
  • ITS region
  • Microcystis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium microcystis aeruginosa in western Japan. / Ohbayashi, Kako; Hodoki, Yoshikuni; Kobayashi, Yuki; Okuda, Noboru; Nakano, Shin Ichi.

In: Canadian Journal of Microbiology, Vol. 59, No. 4, 04.2013, p. 266-272.

Research output: Contribution to journalArticle

Ohbayashi, Kako ; Hodoki, Yoshikuni ; Kobayashi, Yuki ; Okuda, Noboru ; Nakano, Shin Ichi. / Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium microcystis aeruginosa in western Japan. In: Canadian Journal of Microbiology. 2013 ; Vol. 59, No. 4. pp. 266-272.
@article{302524a692a34731ba427bbb6c605dad,
title = "Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium microcystis aeruginosa in western Japan",
abstract = "Microcystis aeruginosa is one of the bloom-forming harmful algae in freshwater ecosystems. We genetically characterized Microcystis populations during bloom-forming periods in various reservoirs, lakes, and ponds in Japan during 2009. Using phylogenetic analysis, we evaluated the relationship between current genotype expansions and geographic location within western Japan and intraspecific variation. Microcystis aeruginosa colonies were isolated at 15 sites and were analyzed by sequencing the 16S-23S internal transcribed spacer (ITS) region of the ribosomal operon, and the potential to produce toxins was assessed by PCR-based detection of the microcystin synthetase gene mcyG. In total, 171 colonies were separated into 41 genotypes. The highest genotypic composition was detected in the south basin of Lake Biwa and the lowest in Lagoon Iba. Cluster analysis indicated no obvious association between genotypic composition and geographic distance. Thus, clear genetic differentiation accompanied by geographic origins was not found in western Japan. The resulting neighbor-joining tree revealed 3 clusters, 2 of which contained strains that showed both nonamplification and amplification of the mcyG gene.",
keywords = "Genotypic diversity, Harmful algae, ITS region, Microcystis",
author = "Kako Ohbayashi and Yoshikuni Hodoki and Yuki Kobayashi and Noboru Okuda and Nakano, {Shin Ichi}",
year = "2013",
month = "4",
doi = "10.1139/cjm-2012-0724",
language = "English",
volume = "59",
pages = "266--272",
journal = "Canadian Journal of Microbiology",
issn = "0008-4166",
publisher = "National Research Council of Canada",
number = "4",

}

TY - JOUR

T1 - Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium microcystis aeruginosa in western Japan

AU - Ohbayashi, Kako

AU - Hodoki, Yoshikuni

AU - Kobayashi, Yuki

AU - Okuda, Noboru

AU - Nakano, Shin Ichi

PY - 2013/4

Y1 - 2013/4

N2 - Microcystis aeruginosa is one of the bloom-forming harmful algae in freshwater ecosystems. We genetically characterized Microcystis populations during bloom-forming periods in various reservoirs, lakes, and ponds in Japan during 2009. Using phylogenetic analysis, we evaluated the relationship between current genotype expansions and geographic location within western Japan and intraspecific variation. Microcystis aeruginosa colonies were isolated at 15 sites and were analyzed by sequencing the 16S-23S internal transcribed spacer (ITS) region of the ribosomal operon, and the potential to produce toxins was assessed by PCR-based detection of the microcystin synthetase gene mcyG. In total, 171 colonies were separated into 41 genotypes. The highest genotypic composition was detected in the south basin of Lake Biwa and the lowest in Lagoon Iba. Cluster analysis indicated no obvious association between genotypic composition and geographic distance. Thus, clear genetic differentiation accompanied by geographic origins was not found in western Japan. The resulting neighbor-joining tree revealed 3 clusters, 2 of which contained strains that showed both nonamplification and amplification of the mcyG gene.

AB - Microcystis aeruginosa is one of the bloom-forming harmful algae in freshwater ecosystems. We genetically characterized Microcystis populations during bloom-forming periods in various reservoirs, lakes, and ponds in Japan during 2009. Using phylogenetic analysis, we evaluated the relationship between current genotype expansions and geographic location within western Japan and intraspecific variation. Microcystis aeruginosa colonies were isolated at 15 sites and were analyzed by sequencing the 16S-23S internal transcribed spacer (ITS) region of the ribosomal operon, and the potential to produce toxins was assessed by PCR-based detection of the microcystin synthetase gene mcyG. In total, 171 colonies were separated into 41 genotypes. The highest genotypic composition was detected in the south basin of Lake Biwa and the lowest in Lagoon Iba. Cluster analysis indicated no obvious association between genotypic composition and geographic distance. Thus, clear genetic differentiation accompanied by geographic origins was not found in western Japan. The resulting neighbor-joining tree revealed 3 clusters, 2 of which contained strains that showed both nonamplification and amplification of the mcyG gene.

KW - Genotypic diversity

KW - Harmful algae

KW - ITS region

KW - Microcystis

UR - http://www.scopus.com/inward/record.url?scp=84876232577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876232577&partnerID=8YFLogxK

U2 - 10.1139/cjm-2012-0724

DO - 10.1139/cjm-2012-0724

M3 - Article

C2 - 23586751

AN - SCOPUS:84876232577

VL - 59

SP - 266

EP - 272

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

IS - 4

ER -