Geranylgeranylacetone protects guinea pig gastric mucosal cells from gastric stressor-induced apoptosis

Tatsunori Takano, Shinji Tsutsumi, Wataru Tomisato, Tatsuya Hoshino, Tomofusa Tsuchiya, Tohru Mizushima

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Various stressors induce apoptosis in gastric mucosal cells, which may cause gastric mucosal lesions in vivo. We recently reproduced gastric stressor-induced apoptosis in vitro, using primary cultures of guinea pig gastric mucosal cells. Geranylgeranylacetone is an antiulcer drug with heat-shock protein-inducing properties. The purpose of this study is to examine the effect of geranylgeranylacetone on gastric stressor-induced apoptosis in vitro. Ethanol, hydrogen peroxide, and hydrochloric acid all induced, in a dose-dependent manner, apoptotic DNA fragmentation. Pretreatment of cells with geranylgeranylacetone inhibited the apoptotic DNA fragmentation caused by each of these gastric stressors. Pretreatment of cells with a low concentration of ethanol, a procedure that is also known to induce heat-shock proteins, made cells resistant to the apoptotic DNA fragmentation. These results suggest that heat-shock proteins could be at least partly involved in the inhibitory effect of geranylgeranylacetone against apoptosis of gastric mucosal cells caused by these gastric stressors.

Original languageEnglish
Pages (from-to)1546-1553
Number of pages8
JournalDigestive Diseases and Sciences
Volume47
Issue number7
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

geranylgeranylacetone
Stomach
Guinea Pigs
Apoptosis
DNA Fragmentation
Heat-Shock Proteins
Ethanol
Hydrochloric Acid
Hydrogen Peroxide

Keywords

  • Apoptosis
  • Gastric mucosal cells
  • Gastric stressors
  • Geranylgeranylacetone
  • Heat-shock proteins

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Geranylgeranylacetone protects guinea pig gastric mucosal cells from gastric stressor-induced apoptosis. / Takano, Tatsunori; Tsutsumi, Shinji; Tomisato, Wataru; Hoshino, Tatsuya; Tsuchiya, Tomofusa; Mizushima, Tohru.

In: Digestive Diseases and Sciences, Vol. 47, No. 7, 2002, p. 1546-1553.

Research output: Contribution to journalArticle

Takano, T, Tsutsumi, S, Tomisato, W, Hoshino, T, Tsuchiya, T & Mizushima, T 2002, 'Geranylgeranylacetone protects guinea pig gastric mucosal cells from gastric stressor-induced apoptosis', Digestive Diseases and Sciences, vol. 47, no. 7, pp. 1546-1553. https://doi.org/10.1023/A:1015819203084
Takano, Tatsunori ; Tsutsumi, Shinji ; Tomisato, Wataru ; Hoshino, Tatsuya ; Tsuchiya, Tomofusa ; Mizushima, Tohru. / Geranylgeranylacetone protects guinea pig gastric mucosal cells from gastric stressor-induced apoptosis. In: Digestive Diseases and Sciences. 2002 ; Vol. 47, No. 7. pp. 1546-1553.
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