GFAP transfected cells produce laminin, leading to neurite outgrowth promotion

Masahiro Toda, Hiroaki Asou, Masayuki Miura, Shigeo Toya, Keiichi Uyemura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Accumulating evidence indicates the importance of astrocytes in neuronal development and regeneration. While glial fibrillary acidic protein (GFAP) is believed to mediate the morphology of developing astrocytes, its precise function remains unknown. To analyse the function of GFAP in astrocytes, we established GFAP-expressing cell lines by transfection of mouse GFAP cDNA into mouse fibroblast L cells. Stable transfectants expressed GFAP uniformly in the cytoplasm with no phenotypic changes and exhibited extended processes rich in GFAP. GFAP-expressing cells significantly promoted the neurite outgrowth of rat cerebral cortex neurones in the co-culture system. Analysis of the products of GFAP-expressing cells revealed an increase in production of laminin, but not fibronectin. These results suggest that L cells expressing GFAP increase laminin production, leading to promotion of neurite outgrowth.

Original languageEnglish
Pages (from-to)1969-1972
Number of pages4
JournalNeuroReport
Volume5
Issue number15
DOIs
Publication statusPublished - 1994 Oct

Keywords

  • Gfap
  • Laminin
  • Neurite outgrowth
  • Regeneration

ASJC Scopus subject areas

  • Neuroscience(all)

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