Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia

Chiemi Miura, Yosuke Shimizu, Maho Uehara, Yuichi Ozaki, Graham Young, Takeshi Miura

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Gh plays important roles in development, somatic growth and gametogenesis in vertebrates. To determine the physiological role of Gh in reproduction in male teleosts, the expression of genes encoding Gh and the two Gh receptors (Ghrs) during spermatogenesis, and the action of Gh in vitro was examined using the Japanese eel (Anguilla japonica). gh, ghr1 and ghr2 mRNA transcripts were detected in all spermatogenic stages. In situ hybridization showed the presence of ghr1 and ghr2 mRNA in the germ cells. Immunohistochemistry using an antiserum against eel Gh indicated that Gh protein was localized to Sertoli cells surrounding the germ cells in early spermatogenesis. Recombinant eel Gh induced spermatogonial proliferation in a testis organ culture system, an effect that was independent from the production of steroid hormones or Igf1. This study identifies a role for eel Gh in the regulation of early spermatogenesis, particularly in the mitotic phase of spermatogenesis, that is not mediated by either steroid hormones or Igf1 production.

Original languageEnglish
Pages (from-to)869-877
Number of pages9
JournalReproduction
Volume142
Issue number6
DOIs
Publication statusPublished - 2011 Dec
Externally publishedYes

Fingerprint

Eels
Spermatogonia
Spermatogenesis
Testis
Germ Cells
Steroids
Hormones
Anguilla
Gametogenesis
Messenger RNA
Sertoli Cells
Organ Culture Techniques
Growth and Development
In Situ Hybridization
Reproduction
Vertebrates
Immune Sera
Immunohistochemistry
Gene Expression
Proteins

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Cite this

Miura, C., Shimizu, Y., Uehara, M., Ozaki, Y., Young, G., & Miura, T. (2011). Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia. Reproduction, 142(6), 869-877. https://doi.org/10.1530/REP-11-0203

Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia. / Miura, Chiemi; Shimizu, Yosuke; Uehara, Maho; Ozaki, Yuichi; Young, Graham; Miura, Takeshi.

In: Reproduction, Vol. 142, No. 6, 12.2011, p. 869-877.

Research output: Contribution to journalArticle

Miura, C, Shimizu, Y, Uehara, M, Ozaki, Y, Young, G & Miura, T 2011, 'Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia', Reproduction, vol. 142, no. 6, pp. 869-877. https://doi.org/10.1530/REP-11-0203
Miura, Chiemi ; Shimizu, Yosuke ; Uehara, Maho ; Ozaki, Yuichi ; Young, Graham ; Miura, Takeshi. / Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia. In: Reproduction. 2011 ; Vol. 142, No. 6. pp. 869-877.
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