Glioma cell extracellular matrix metalloproteinase inducer (EMMPRIN) (CD147) stimulates production of membrane-type matrix metalloproteinases and activated gelatinase A in co-cultures with brain-derived fibroblasts

Tetsuro Sameshima, Kazuki Nabeshima, Bryan P. Toole, Kiyotaka Yokogami, Yasunori Okada, Tomokazu Goya, Masashi Koono, Shinichiro Wakisaka

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Extracellular matrix metalloproteinase inducer (EMMPRIN) also called CD147, basigin or M6 in the human is a member of the immunoglobulin superfamily that is enriched on the surface of tumor cells and stimulates adjacent stromal cells to produce several matrix metalloproteinases (MMPs). In this study, we have demonstrated that coculturing of EMMPRIN-expressing human glioblastoma multiforme cells (U251) with brain-derived human fibroblasts not only stimulates production, but also activation of pro- gelatinase A (proMMP-2), an enzyme that is enriched in malignant gliomas and most likely crucial to tumor progression. Production of membrane types 1 and 2-MMPs (MT1-MMP and MT2-MMP), which are activators of proMMP-2, was also stimulated in these cocultures. Stimulation of MMP-2, MT1-MMP and MT2-MMP production was inhibited by anti-EMMPRIN monoclonal antibody in a dose- dependent manner. Thus, we have shown, for the first time, that EMMPRIN causes increased expression of MT1-MMP and MT2-MMP, as well as increased production and activation of MMP-2. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)177-184
Number of pages8
JournalCancer Letters
Volume157
Issue number2
DOIs
Publication statusPublished - 2000 Sep 1

    Fingerprint

Keywords

  • Extracellular matrix metalloproteinase inducer/basigin
  • Gelatinase A
  • Glioma
  • Matrix metalloproteinases
  • Membrane-type matrix metalloproteinases

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this