Global metabolic network reorganization by adaptive mutations allows fast growth of Escherichia coli on glycerol

Kian Kai Cheng, Baek Seok Lee, Takeshi Masuda, Takuro Ito, Kazutaka Ikeda, Akiyoshi Hirayama, Lingli Deng, Jiyang Dong, Kazuyuki Shimizu, Tomoyoshi Soga, Masaru Tomita, Bernhard O. Palsson, Martin Robert

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Comparative whole-genome sequencing enables the identification of specific mutations during adaptation of bacteria to new environments and allelic replacement can establish their causality. However, the mechanisms of action are hard to decipher and little has been achieved for epistatic mutations, especially at the metabolic level. Here we show that a strain of Escherichia coli carrying mutations in the rpoC and glpK genes, derived from adaptation in glycerol, uses two distinct metabolic strategies to gain growth advantage. A 27-bp deletion in the rpoC gene first increases metabolic efficiency. Then, a point mutation in the glpK gene promotes growth by improving glycerol utilization but results in increased carbon wasting as overflow metabolism. In a strain carrying both mutations, these contrasting carbon/energy saving and wasting mechanisms work together to give an 89% increase in growth rate. This study provides insight into metabolic reprogramming during adaptive laboratory evolution for fast cellular growth.

Original languageEnglish
Article number3233
JournalNature communications
Volume5
DOIs
Publication statusPublished - 2014 Jan 31

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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    Cheng, K. K., Lee, B. S., Masuda, T., Ito, T., Ikeda, K., Hirayama, A., Deng, L., Dong, J., Shimizu, K., Soga, T., Tomita, M., Palsson, B. O., & Robert, M. (2014). Global metabolic network reorganization by adaptive mutations allows fast growth of Escherichia coli on glycerol. Nature communications, 5, [3233]. https://doi.org/10.1038/ncomms4233