Global protein-expression analysis of bone and soft tissue sarcomas

Akira Kawai, Tadashi Kondo, Yoshiyuki Suehara, Kazutaka Kikuta, Setsuo Hirohashi

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Analysis of global protein expression, an approach known as expression proteomics, can offer important clues for understanding tumor biology that cannot be obtained by other approaches (e.g., genome or transcriptome analysis). Using two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry, we performed global protein expression studies of bone and soft tissue sarcomas to develop novel diagnostic and therapeutic biomarkers and allow molecular classification of the tumors. Among 1500 protein variants identified in the two-dimensional gel, 67 proteins correctly distinguished the eight subtypes of 99 histologically classified soft tissue sarcomas. Hierarchical clustering demonstrated leiomyosarcoma and MFH shared a similar protein expression profile, and clear cell sarcoma, synovial sarcoma, and MPNST could be grouped according to their protein expression patterns. Pleomorphic leiomyosarcoma and MFH showed similar tropomyosin isoform expression patterns. Patients with gastrointestinal stromal tumors expressing pfetin protein had better survival than those whose tumors lacked it. We identified 10 protein spots associated with the chemosensitivity of osteosarcoma to preoperative chemotherapy. These 10 spots could be new diagnostic and prognostic markers for osteosarcoma and new therapeutic targets for the disease. Proteomic analysis using 2D-DIGE provides novel information on the biology of bone and soft tissue sarcomas that could be used to diagnosis and treat these tumors. Level of Evidence: Level II, diagnostic study. See the Guidelines for Authors for a complete description of levels of evidence.

Original languageEnglish
Pages (from-to)2099-2106
Number of pages8
JournalClinical Orthopaedics and Related Research
Volume466
Issue number9
DOIs
Publication statusPublished - 2008 Sep
Externally publishedYes

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Sarcoma
Bone and Bones
Two-Dimensional Difference Gel Electrophoresis
Proteins
Leiomyosarcoma
Osteosarcoma
Proteomics
Neoplasms
Clear Cell Sarcoma
Synovial Sarcoma
Tropomyosin
Gastrointestinal Stromal Tumors
Neurilemmoma
Gene Expression Profiling
Cluster Analysis
Mass Spectrometry
Protein Isoforms
Biomarkers
Gels
Genome

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Global protein-expression analysis of bone and soft tissue sarcomas. / Kawai, Akira; Kondo, Tadashi; Suehara, Yoshiyuki; Kikuta, Kazutaka; Hirohashi, Setsuo.

In: Clinical Orthopaedics and Related Research, Vol. 466, No. 9, 09.2008, p. 2099-2106.

Research output: Contribution to journalArticle

Kawai, A, Kondo, T, Suehara, Y, Kikuta, K & Hirohashi, S 2008, 'Global protein-expression analysis of bone and soft tissue sarcomas', Clinical Orthopaedics and Related Research, vol. 466, no. 9, pp. 2099-2106. https://doi.org/10.1007/s11999-008-0330-4
Kawai, Akira ; Kondo, Tadashi ; Suehara, Yoshiyuki ; Kikuta, Kazutaka ; Hirohashi, Setsuo. / Global protein-expression analysis of bone and soft tissue sarcomas. In: Clinical Orthopaedics and Related Research. 2008 ; Vol. 466, No. 9. pp. 2099-2106.
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