Abstract
Analysis of global protein expression, an approach known as expression proteomics, can offer important clues for understanding tumor biology that cannot be obtained by other approaches (e.g., genome or transcriptome analysis). Using two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry, we performed global protein expression studies of bone and soft tissue sarcomas to develop novel diagnostic and therapeutic biomarkers and allow molecular classification of the tumors. Among 1500 protein variants identified in the two-dimensional gel, 67 proteins correctly distinguished the eight subtypes of 99 histologically classified soft tissue sarcomas. Hierarchical clustering demonstrated leiomyosarcoma and MFH shared a similar protein expression profile, and clear cell sarcoma, synovial sarcoma, and MPNST could be grouped according to their protein expression patterns. Pleomorphic leiomyosarcoma and MFH showed similar tropomyosin isoform expression patterns. Patients with gastrointestinal stromal tumors expressing pfetin protein had better survival than those whose tumors lacked it. We identified 10 protein spots associated with the chemosensitivity of osteosarcoma to preoperative chemotherapy. These 10 spots could be new diagnostic and prognostic markers for osteosarcoma and new therapeutic targets for the disease. Proteomic analysis using 2D-DIGE provides novel information on the biology of bone and soft tissue sarcomas that could be used to diagnosis and treat these tumors. Level of Evidence: Level II, diagnostic study. See the Guidelines for Authors for a complete description of levels of evidence.
| Original language | English |
|---|---|
| Pages (from-to) | 2099-2106 |
| Number of pages | 8 |
| Journal | Clinical Orthopaedics and Related Research |
| Volume | 466 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2008 Sep |
| Externally published | Yes |
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ASJC Scopus subject areas
- Orthopedics and Sports Medicine
- Surgery
Cite this
Global protein-expression analysis of bone and soft tissue sarcomas. / Kawai, Akira; Kondo, Tadashi; Suehara, Yoshiyuki; Kikuta, Kazutaka; Hirohashi, Setsuo.
In: Clinical Orthopaedics and Related Research, Vol. 466, No. 9, 09.2008, p. 2099-2106.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Global protein-expression analysis of bone and soft tissue sarcomas
AU - Kawai, Akira
AU - Kondo, Tadashi
AU - Suehara, Yoshiyuki
AU - Kikuta, Kazutaka
AU - Hirohashi, Setsuo
PY - 2008/9
Y1 - 2008/9
N2 - Analysis of global protein expression, an approach known as expression proteomics, can offer important clues for understanding tumor biology that cannot be obtained by other approaches (e.g., genome or transcriptome analysis). Using two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry, we performed global protein expression studies of bone and soft tissue sarcomas to develop novel diagnostic and therapeutic biomarkers and allow molecular classification of the tumors. Among 1500 protein variants identified in the two-dimensional gel, 67 proteins correctly distinguished the eight subtypes of 99 histologically classified soft tissue sarcomas. Hierarchical clustering demonstrated leiomyosarcoma and MFH shared a similar protein expression profile, and clear cell sarcoma, synovial sarcoma, and MPNST could be grouped according to their protein expression patterns. Pleomorphic leiomyosarcoma and MFH showed similar tropomyosin isoform expression patterns. Patients with gastrointestinal stromal tumors expressing pfetin protein had better survival than those whose tumors lacked it. We identified 10 protein spots associated with the chemosensitivity of osteosarcoma to preoperative chemotherapy. These 10 spots could be new diagnostic and prognostic markers for osteosarcoma and new therapeutic targets for the disease. Proteomic analysis using 2D-DIGE provides novel information on the biology of bone and soft tissue sarcomas that could be used to diagnosis and treat these tumors. Level of Evidence: Level II, diagnostic study. See the Guidelines for Authors for a complete description of levels of evidence.
AB - Analysis of global protein expression, an approach known as expression proteomics, can offer important clues for understanding tumor biology that cannot be obtained by other approaches (e.g., genome or transcriptome analysis). Using two-dimensional difference gel electrophoresis (2D-DIGE) and mass spectrometry, we performed global protein expression studies of bone and soft tissue sarcomas to develop novel diagnostic and therapeutic biomarkers and allow molecular classification of the tumors. Among 1500 protein variants identified in the two-dimensional gel, 67 proteins correctly distinguished the eight subtypes of 99 histologically classified soft tissue sarcomas. Hierarchical clustering demonstrated leiomyosarcoma and MFH shared a similar protein expression profile, and clear cell sarcoma, synovial sarcoma, and MPNST could be grouped according to their protein expression patterns. Pleomorphic leiomyosarcoma and MFH showed similar tropomyosin isoform expression patterns. Patients with gastrointestinal stromal tumors expressing pfetin protein had better survival than those whose tumors lacked it. We identified 10 protein spots associated with the chemosensitivity of osteosarcoma to preoperative chemotherapy. These 10 spots could be new diagnostic and prognostic markers for osteosarcoma and new therapeutic targets for the disease. Proteomic analysis using 2D-DIGE provides novel information on the biology of bone and soft tissue sarcomas that could be used to diagnosis and treat these tumors. Level of Evidence: Level II, diagnostic study. See the Guidelines for Authors for a complete description of levels of evidence.
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UR - http://www.scopus.com/inward/citedby.url?scp=50649085034&partnerID=8YFLogxK
U2 - 10.1007/s11999-008-0330-4
DO - 10.1007/s11999-008-0330-4
M3 - Article
C2 - 18535868
AN - SCOPUS:50649085034
VL - 466
SP - 2099
EP - 2106
JO - Clinical Orthopaedics and Related Research
JF - Clinical Orthopaedics and Related Research
SN - 0009-921X
IS - 9
ER -