Glucan synthase-like 2 is indispensable for paramylon synthesis in Euglena gracilis

Yuji Tanaka, Takahisa Ogawa, Takanori Maruta, Yuta Yoshida, Kazuharu Arakawa, Takahiro Ishikawa

Research output: Contribution to journalLetter

15 Citations (Scopus)

Abstract

The phytoflagellate Euglena gracilis produces a large amount of paramylon (PM), a conglomerate of liner β-1,3-glucan chains, as a storage polysaccharide. PM is synthesized from uridine diphosphate-glucose, but its mechanism of formation is largely unknown. Two enzymes, glucan synthase-like (EgGSL) 1 and EgGSL2 were previously identified as candidates for PM synthesis in a Euglena transcriptome analysis. Here, we performed a reverse genetic analysis on these enzymes. Knockdown of EgGSL2, but not EgGSL1, significantly inhibits PM accumulation in Euglena cells. Additionally, β-1,3-glucan synthesis is detected in a PM-associated membrane fraction extracted from Euglena cells. Our findings indicate that EgGSL2 is the predominant enzyme for PM biosynthesis.

Original languageEnglish
Pages (from-to)1360-1370
Number of pages11
JournalFEBS Letters
Volume591
Issue number10
DOIs
Publication statusPublished - 2017 May 1

Fingerprint

Euglena
Glucans
Enzymes
Uridine Diphosphate Glucose
Reverse Genetics
Biosynthesis
Gene Expression Profiling
Polysaccharides
glucan synthase
paramylon
Membranes

Keywords

  • Euglena gracilis
  • paramylon
  • β-1,3-glucan synthase

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Glucan synthase-like 2 is indispensable for paramylon synthesis in Euglena gracilis. / Tanaka, Yuji; Ogawa, Takahisa; Maruta, Takanori; Yoshida, Yuta; Arakawa, Kazuharu; Ishikawa, Takahiro.

In: FEBS Letters, Vol. 591, No. 10, 01.05.2017, p. 1360-1370.

Research output: Contribution to journalLetter

Tanaka, Y, Ogawa, T, Maruta, T, Yoshida, Y, Arakawa, K & Ishikawa, T 2017, 'Glucan synthase-like 2 is indispensable for paramylon synthesis in Euglena gracilis', FEBS Letters, vol. 591, no. 10, pp. 1360-1370. https://doi.org/10.1002/1873-3468.12659
Tanaka, Yuji ; Ogawa, Takahisa ; Maruta, Takanori ; Yoshida, Yuta ; Arakawa, Kazuharu ; Ishikawa, Takahiro. / Glucan synthase-like 2 is indispensable for paramylon synthesis in Euglena gracilis. In: FEBS Letters. 2017 ; Vol. 591, No. 10. pp. 1360-1370.
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