Glucocorticoids stimulate the maturation of H,K-ATPase in the infant rat stomach

Zheng Ming Wang, Roman Aizman, Lena Grahnquist, Masato Yasui, Åsa Hemphälä, Gianni Celsi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The development of gastric H,K-ATPase from fetal to adult life was studied in the rat. The α and β H,K-ATPase mRNA abundance, the protein abundance, and the enzyme activity increased postnatally. The sharpest increase in mRNA and enzyme activity was observed in the weaning period. Several intestinal enzymes are known to be stimulated by glucocorticoids at the time of weaning. To study the role of glucocorticoids in the maturation of gastric H,K-ATPase, we treated 10-d-old rats with a single injection of betamethasone. Twenty-four hours after betamethasone injection, the enzyme activity was significantly higher than in the control animals (2.6-fold, p < 0.05). The abundance of catalytic α H,K-ATPase protein was also increased (2.5-fold, p < 0.01). The time-dependent effect of betamethasone on α H,K- ATPase mRNA was determined from 6 to 24 h after treatment. Glucocorticoids did not significantly alter the mRNA abundance within 18 h. Twenty four hours after injection, the gastric H,K-ATPase mRNA was significantly increased compared with controls (2.8- and 2.2-fold increase for α and β subunits, respectively, p < 0.01 for both). In conclusion this study indicates that glucocorticoids may regulate the long term maturation of gastric H,K-ATPase by indirectly stimulating enzyme synthesis.

Original languageEnglish
Pages (from-to)658-663
Number of pages6
JournalPediatric Research
Volume40
Issue number5
Publication statusPublished - 1996 Nov
Externally publishedYes

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H(+)-K(+)-Exchanging ATPase
Proton-Translocating ATPases
Glucocorticoids
Stomach
Betamethasone
Messenger RNA
Enzymes
Weaning
Injections
Proteins

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Wang, Z. M., Aizman, R., Grahnquist, L., Yasui, M., Hemphälä, Å., & Celsi, G. (1996). Glucocorticoids stimulate the maturation of H,K-ATPase in the infant rat stomach. Pediatric Research, 40(5), 658-663.

Glucocorticoids stimulate the maturation of H,K-ATPase in the infant rat stomach. / Wang, Zheng Ming; Aizman, Roman; Grahnquist, Lena; Yasui, Masato; Hemphälä, Åsa; Celsi, Gianni.

In: Pediatric Research, Vol. 40, No. 5, 11.1996, p. 658-663.

Research output: Contribution to journalArticle

Wang, ZM, Aizman, R, Grahnquist, L, Yasui, M, Hemphälä, Å & Celsi, G 1996, 'Glucocorticoids stimulate the maturation of H,K-ATPase in the infant rat stomach', Pediatric Research, vol. 40, no. 5, pp. 658-663.
Wang ZM, Aizman R, Grahnquist L, Yasui M, Hemphälä Å, Celsi G. Glucocorticoids stimulate the maturation of H,K-ATPase in the infant rat stomach. Pediatric Research. 1996 Nov;40(5):658-663.
Wang, Zheng Ming ; Aizman, Roman ; Grahnquist, Lena ; Yasui, Masato ; Hemphälä, Åsa ; Celsi, Gianni. / Glucocorticoids stimulate the maturation of H,K-ATPase in the infant rat stomach. In: Pediatric Research. 1996 ; Vol. 40, No. 5. pp. 658-663.
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