GLUT6 is a lysosomal transporter that is regulated by inflammatory stimuli and modulates glycolysis in macrophages

Shotaro Maedera, Tadahaya Mizuno, Hiromu Ishiguro, Takuya Ito, Tomoyoshi Soga, Hiroyuki Kusuhara

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The solute carrier family is an important protein class governing compound transport across membranes. However, some of its members remain functionally unidentified. We analyzed ChIP-seq data for the NF-κB family transcription factor RelA and identified GLUT6 as a functionally uncharacterized transporter that putatively works in inflammatory responses. Inflammatory stimuli increase GLUT6 expression level, although GLUT6-knockout mice exhibit a subtle phenotype to lipopolysaccharide administration. Metabolomics and in vitro analyses show that GLUT6 functions as a glycolysis modulator in inflammatory macrophages. GLUT6 does not mediate glucose uptake and is localized on lysosomal membranes. We conclude that GLUT6 is a lysosomal transporter that is regulated by inflammatory stimuli and modulates inflammatory responses by affecting the metabolic shift in macrophages.

Original languageEnglish
Pages (from-to)195-208
Number of pages14
JournalFEBS Letters
Volume593
Issue number2
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Macrophages
Glycolysis
Transcription Factor RelA
Membranes
Metabolomics
Knockout Mice
Modulators
Lipopolysaccharides
Phenotype
Glucose
Proteins

Keywords

  • GLUT6
  • inflammation
  • macrophages

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

GLUT6 is a lysosomal transporter that is regulated by inflammatory stimuli and modulates glycolysis in macrophages. / Maedera, Shotaro; Mizuno, Tadahaya; Ishiguro, Hiromu; Ito, Takuya; Soga, Tomoyoshi; Kusuhara, Hiroyuki.

In: FEBS Letters, Vol. 593, No. 2, 01.01.2019, p. 195-208.

Research output: Contribution to journalArticle

Maedera, Shotaro ; Mizuno, Tadahaya ; Ishiguro, Hiromu ; Ito, Takuya ; Soga, Tomoyoshi ; Kusuhara, Hiroyuki. / GLUT6 is a lysosomal transporter that is regulated by inflammatory stimuli and modulates glycolysis in macrophages. In: FEBS Letters. 2019 ; Vol. 593, No. 2. pp. 195-208.
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