Glutathione isopropyl ester reduces UVB-induced skin damage in hairless mice

Shizuko Kobayashi, Makoto Takehana, Chiharu Tohyama

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The protective effect of administration of glutathione (GSH) isopropyl ester on photodamage, such as lipid peroxidation, inflammation and tumorigenesis induced by UV exposure (290-400 nm, max. 312 nm), was investigated using hairless mice. Pretreatment with 20 mg/kg GSH isopropyl ester prevented the increases of thiobarbituric acid-reactive substance (TBARS) formation in skin and serum sialic acid, indices of lipid peroxidation and inflammatory reaction, respectively, which were caused by a single dose (15 kJ/m2) of UV irradiation. The level of epidermal GSH in skins of the GSH ester-treated mice was maintained within normal limits. When mice were exposed to UV at a dose of 2 kJ/m2, three times weekly, skin tumors developed in all of them after 25 weeks. The formation of skin tumors was significantly inhibited by administration of 10 mg/kg GSH ester prior to each UV irradiation for 25 weeks. Moreover, the increases of cutaneous TBARS and serum sialic acid in the tumor-bearing mice were also prevented by continuous pretreatment with GSH ester. Even after 24 weeks, the epidermal GSH content of the pretreated mice was mostly retained compared to nonirradiated mice. However, administration of GSH prior to acute or chronic UV irradiation had no effect on the UV-induced damage. The present results suggest that the protection from photodamage afforded by pretreatment with GSH ester is due to maintenance of a normal GSH level.

Original languageEnglish
Pages (from-to)106-110
Number of pages5
JournalPhotochemistry and Photobiology
Volume63
Issue number1
DOIs
Publication statusPublished - 1996 Jan

Fingerprint

Hairless Mouse
glutathione
mice
esters
Skin
Esters
damage
pretreatment
Thiobarbituric Acid Reactive Substances
Tumors
N-Acetylneuraminic Acid
acids
tumors
Lipid Peroxidation
Irradiation
serums
irradiation
lipids
Bearings (structural)
Neoplasms

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Biochemistry
  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biophysics

Cite this

Glutathione isopropyl ester reduces UVB-induced skin damage in hairless mice. / Kobayashi, Shizuko; Takehana, Makoto; Tohyama, Chiharu.

In: Photochemistry and Photobiology, Vol. 63, No. 1, 01.1996, p. 106-110.

Research output: Contribution to journalArticle

Kobayashi, Shizuko ; Takehana, Makoto ; Tohyama, Chiharu. / Glutathione isopropyl ester reduces UVB-induced skin damage in hairless mice. In: Photochemistry and Photobiology. 1996 ; Vol. 63, No. 1. pp. 106-110.
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