Glycoconjugate profiles of insect spermatogenesis: Lectin cytochemical analysis of the cricket, Gryllus bimaculatus

Atsushi C Suzuki, Kenji Nishimura

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3 Citations (Scopus)

Abstract

The distribution of glycoconjugate in testicular germ cells of the cricket, Gryllus bimaculatus, was cytochemically investigated using a panel of lectins and several kinds of glycosidases. Observations were focused on the early process of spermatogenesis. The binding pattern with N-acetylgalactosamine-binding lectins, especially with Dolichos biflorus agglutinin, and their susceptibility to peptide-N-glycosidase F (PNGase F) suggested the stage-specific expression of N-linked glycoproteins with terminal α-N-acetylgalactosamine in the early meiotic prophase. In the primary spermatocyte, the transitory expression of PNGase F-resistant glycoproteins was detected by several mannose-binding and fucose-binding lectins such as Lens culinaris agglutinin and Anguilla anguilla agglutinin. Hyaluronidase-sensitive glycoconjugates also distributed widely in cricket testes, exemplified by perinuclear granular structures recognized by Helix pomatia agglutinin and nuclear staining with Phaseolus lunatus agglutinin. These lectin-binding affinities in meiotic prophase were discussed in reference to many informations about carbohydrate-lectin binding specificities.

Original languageEnglish
Pages (from-to)455-467
Number of pages13
JournalZoological Science
Volume14
Issue number3
Publication statusPublished - 1997 Jun

Fingerprint

Gryllus bimaculatus
glycoconjugates
agglutinins
Gryllidae
spermatogenesis
lectins
glycosidases
insects
prophase
glycoproteins
Dolichos
peptides
Helix pomatia
Phaseolus lunatus
hyaluronoglucosaminidase
fucose
Lens culinaris
spermatocytes
Anguilla anguilla
mannose

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Glycoconjugate profiles of insect spermatogenesis : Lectin cytochemical analysis of the cricket, Gryllus bimaculatus. / Suzuki, Atsushi C; Nishimura, Kenji.

In: Zoological Science, Vol. 14, No. 3, 06.1997, p. 455-467.

Research output: Contribution to journalArticle

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