Glycogen is the primary source of glucose during the lag phase of E. coli proliferation

Tomoaki Yamamotoya, Hitomi Dose, Zhongyuan Tian, Adrien Fauré, Yoshihiro Toya, Masayuki Honma, Kaori Igarashi, Kenji Nakahigashi, Tomoyoshi Soga, Hirotada Mori, Hiroshi Matsuno

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In the studies of Escherichia coli (E. coli), metabolomics analyses have mainly been performed using steady state culture. However, to analyze the dynamic changes in cellular metabolism, we performed a profiling of concentration of metabolites by using batch culture. As a first step, we focused on glucose uptake and the behavior of the first metabolite, G6P (glucose-6-phosphate). A computational formula was derived to express the glucose uptake rate by a single cell from two kinds of experimental data, extracellular glucose concentration and cell growth, being simulated by Cell Illustrator. In addition, average concentration of G6P has been measured by CE-MS. The existence of another carbon source was suggested from the computational result. After careful comparison between cell growth, G6P concentration, and the computationally obtained curve of glucose uptake rate, we predicted the consumption of glycogen in lag phase and its accumulation as an energy source in an E. coli cell for the next proliferation. We confirmed our prediction experimentally. This behavior indicates the importance of glycogen participation in the lag phase for the growth of E. coli. This article is part of a Special Issue entitled: Computational Methods for Protein Interaction and Structural Prediction.

Original languageEnglish
Pages (from-to)1442-1448
Number of pages7
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1824
Issue number12
DOIs
Publication statusPublished - 2012 Dec

Fingerprint

Glycogen
Glucose-6-Phosphate
Escherichia coli
Glucose
Cell growth
Metabolites
Growth
Batch Cell Culture Techniques
Metabolomics
Computational methods
Metabolism
Carbon
Cell Proliferation
Proteins

Keywords

  • Cell growth
  • Computational simulation
  • E. coli metabolism
  • Glycogen

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Analytical Chemistry
  • Molecular Biology

Cite this

Glycogen is the primary source of glucose during the lag phase of E. coli proliferation. / Yamamotoya, Tomoaki; Dose, Hitomi; Tian, Zhongyuan; Fauré, Adrien; Toya, Yoshihiro; Honma, Masayuki; Igarashi, Kaori; Nakahigashi, Kenji; Soga, Tomoyoshi; Mori, Hirotada; Matsuno, Hiroshi.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1824, No. 12, 12.2012, p. 1442-1448.

Research output: Contribution to journalArticle

Yamamotoya, T, Dose, H, Tian, Z, Fauré, A, Toya, Y, Honma, M, Igarashi, K, Nakahigashi, K, Soga, T, Mori, H & Matsuno, H 2012, 'Glycogen is the primary source of glucose during the lag phase of E. coli proliferation', Biochimica et Biophysica Acta - Proteins and Proteomics, vol. 1824, no. 12, pp. 1442-1448. https://doi.org/10.1016/j.bbapap.2012.06.010
Yamamotoya, Tomoaki ; Dose, Hitomi ; Tian, Zhongyuan ; Fauré, Adrien ; Toya, Yoshihiro ; Honma, Masayuki ; Igarashi, Kaori ; Nakahigashi, Kenji ; Soga, Tomoyoshi ; Mori, Hirotada ; Matsuno, Hiroshi. / Glycogen is the primary source of glucose during the lag phase of E. coli proliferation. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2012 ; Vol. 1824, No. 12. pp. 1442-1448.
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