TY - JOUR
T1 - Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells
AU - Ojima, Takuma
AU - Shibata, Eri
AU - Saito, Shiho
AU - Toyoda, Masashi
AU - Nakajima, Hideki
AU - Yamazaki-Inoue, Mayu
AU - Miyagawa, Yoshitaka
AU - Kiyokawa, Nobutaka
AU - Fujimoto, Jun Ichiro
AU - Sato, Toshinori
AU - Umezawa, Akihiro
N1 - Funding Information:
We would like to express our sincere thanks to K. Iijima for providing expert technical assistance, K. Minami for the support throughout the work, Dr. C. Ketcham for English editing and proofreading, and E. Suzuki, Y. Suehiro, and K. Saito for secretarial work. This research was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan; by Ministry of Health, Labour and Welfare Sciences (MHLW) research grants; by a Research Grant on Health Science focusing on Drug Innovation from the Japan Health Science Foundation; by the program for the promotion of Fundamental Studies in Health Science of the Pharmaceuticals and Medical Devices Agency; by the Grant of National Center for Child Health and Development; by Research Seeds Quest Program from the Japan Science and Technology Agency (JST). We acknowledge the International High Cited Research Group (IHCRG #14-104), Deanship of Scientific Research, King Saudi University, Riyadh, Kingdom of Saudi Arabia. AU thanks King Saud University, Riyadh, Kingdom of Saudi Arabia, for the Visiting Professorship.
PY - 2015/10/19
Y1 - 2015/10/19
N2 - Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells €(tm) genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells.
AB - Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells €(tm) genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells.
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U2 - 10.1038/srep14988
DO - 10.1038/srep14988
M3 - Article
C2 - 26477663
AN - SCOPUS:84945189159
SN - 2045-2322
VL - 5
JO - Scientific Reports
JF - Scientific Reports
M1 - 14988
ER -