Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells

Takuma Ojima, Eri Shibata, Shiho Saito, Masashi Toyoda, Hideki Nakajima, Mayu Yamazaki-Inoue, Yoshitaka Miyagawa, Nobutaka Kiyokawa, Jun Ichiro Fujimoto, Toshinori Sato, Akihiro Umezawa

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15 Citations (Scopus)

Abstract

Glycosphingolipids (GSLs) are glycoconjugates that function as mediators of cell adhesion and modulators of signal transduction. Some well-defined markers of undifferentiated human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are glycoconjugates, such as SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81. However, Comprehensive GSL profiles of hiPSCs have not yet been elucidated. The global images of GSLs from the parental cells, hiPSCs, and differentiated cells revealed that there are parental cell-independent specific glycolipids, including Globo H (fucosyl-Gb5Cer) and H type1 antigen (fucosyl-Lc4Cer) that are novel markers for undifferentiated hiPSCs. Interestingly, undifferentiated hiPSCs expressed H type 1 antigen, specific for blood type O, regardless of the cells €(tm) genotypes. Thus, in this study, we defined the dynamics of GSL remodeling during reprogramming from parental cell sets to iPSC sets and thence to iPSC-neural cells.

Original languageEnglish
Article number14988
JournalScientific Reports
Volume5
DOIs
Publication statusPublished - 2015 Oct 19

ASJC Scopus subject areas

  • General

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