Glycoprotein Ib-von Willebrand factor interactions activate tyrosine kinases in human platelets

Naoki Asazuma, Yukio Ozaki, Kaneo Satoh, Yutaka Yatomi, Makoto Handa, Yoshihiro Fujimura, Shuji Miura, Shoji Kume

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77 Citations (Scopus)

Abstract

von Willebrand factor (vWF) in the presence of botrocetin induces p72(syk) activation, assessed as its autophosphorylated level and in vitro kinase assays, the transient association of p72(syk) with p60(c-src), and the translocation of p60(c-src) and p54/58(lyn) to cytoskeletal fractions. Jararaca glycoprotein lb-binding protein (GPIb-BP), which specifically binds to GPIb, abolished these phenomena, suggesting that they are mediated by the vWF-GPIb interaction. These tyrosine kinase-related events were not inhibited by GRGDS peptide (plus EGTA), indicating that GPIIb/IIIa is not involved in the observed responses. Shc, an adaptor protein, was also tyrosine phosphorylated by the botrocetin-vWF activation. When GPIb was immunoprecipitated with nonfunctional monoclonal antibodies (MoAbs) directed against GPIb, a kinase activity was found to associate with GPIb upon botrocetin-vWF activation. On the other hand, anti-GPIb MoAbs that inhibit the vWF-GPIb interaction did not coprecipitate a kinase activity. Because the recovery of GPIb did not differ significantly, it is suggested that the excessive presence of inhibitory anti-GPIb MoAb dissociated a kinase activity from GPIb. Phosphoamino acid analysis showed that the kinase activity was that of a tyrosine kinase. The identity of the tyrosine kinase end the mode of interaction with the cytoplasmic region of GPIb await to be determined. Our findings suggest that the tyrosine kinase associated with GPIb serves at a most proximal step in the signal transduction pathway involved in the vWF- GPIb-induced platelet activation, which leads to other tyrosine kinase- related intracellular signals.

Original languageEnglish
Pages (from-to)4789-4798
Number of pages10
JournalBlood
Volume90
Issue number12
DOIs
Publication statusPublished - 1997 Dec 15

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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    Asazuma, N., Ozaki, Y., Satoh, K., Yatomi, Y., Handa, M., Fujimura, Y., Miura, S., & Kume, S. (1997). Glycoprotein Ib-von Willebrand factor interactions activate tyrosine kinases in human platelets. Blood, 90(12), 4789-4798. https://doi.org/10.1182/blood.v90.12.4789.4789_4789_4798