Glycyrrhiza inflata-derived chalcones, Licochalcone A, Licochalcone B and Licochalcone D, inhibit phosphorylation of NF-κB p65 in LPS signaling pathway

Jun ichi Furusawa, Megumi Funakoshi-Tago, Tadahiko Mashino, Kenji Tago, Hideo Inoue, Yoshiko Sonoda, Tadashi Kasahara

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Licorice root has been used as a traditional medicine for the treatment of gastric ulcer, bronchial asthma and inflammation. Licochalcone A is a major component of Xinjiang licorice, Glycyrrhiza inflata. Previously we showed that Licochalcone A significantly inhibited LPS-induced NF-κB transcriptional activation by abrogating the phosphorylation of NF-κB p65 at serine 276. Glycyrrhiza inflata contains not only Licochalcone A but also Licochalcone B, Licochalcone C, Licochalcone D, Echinatin and Isoliquiritigenin, harboring the common structure of chalcones. No chalcones had any effect on LPS-induced IκB degradation, nuclear translocation and DNA binding activity of NF-κB p65; however, we observed that Licochalcone B and Licochalcone D significantly inhibited LPS-induced phosphorylation at serine 276 and transcriptional activation of NF-κB, the same as Licochalcone A. Interestingly, we also found that Licochalcone A, Licochalcone B and Licochalcone D effectively inhibited LPS-induced activation of PKA, which is required for the phosphorylation of NF-κB p65 at serine 276. Consequently, Licochalcone B and Licochalcone D significantly reduced the LPS-induced production of NO, TNFα and MCP-1. On the other hand, Licochalcone C, Echinatin and Isoliquitigenin failed to inhibit LPS-induced NF-κB activation. These findings suggest that the anti-inflammatory effect of Glycyrrhiza inflata is ascribable to the potent inhibition of NF-κB by Licochalcone A, Licochalcone B and Licochalcone D.

Original languageEnglish
Pages (from-to)499-507
Number of pages9
JournalInternational Immunopharmacology
Volume9
Issue number4
DOIs
Publication statusPublished - 2009 Apr 1

Keywords

  • Glycyrrhiza inflata
  • LPS
  • Licochalcone A
  • Licochalcone B
  • Licochalcone D
  • NF-κB

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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