GM3 signals regulating TNF-alpha expression are mediated by rictor and Arhgdib in mouse melanoma B16 cells

Pu Wang, Xiaoyan Yang, Peixing Wu, Jinghai Zhang, Toshinori Sato, Sadako Yamagata, Tatsuya Yamagata

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: We have previously shown GM3 to positively regulate TNF-α expression via a PI3K/Akt pathway in mouse melanoma B16 cells [Wang et al.: Biochem Biophys Res Commun 2007;356:438-443]. The GM3 signal was shown to be located upstream of Akt, but whether it is located upstream of PI3K and which molecule is the effector of PI3K remain to be clarified. Methods: We used inhibitors of PI3K and mTOR, and siRNA directed to Rictor, Raptor and Rho-GDP dissociation inhibitor β (Arhgdib). Results: PI3K inhibitors LY294002 and LY303511 were shown to suppress TNF-α expression that is stimulated by GM3 in B16 cells, suggesting that the GM3 signal is located upstream of the PI3K-Akt pathway. Rapamycin suppressed TNF-α expression, indicating mTOR to be involved in the pathway. Either siRNA Raptor or siRNA Rictor suppressed TNF-α expression, but the latter suppressed the effects of GM3 on TNF-α expression and Akt phosphorylation at Ser473, indicating the GM3 signal to be transduced via Rictor/mTOR and Akt (Ser473), leading to TNF-α stimulation. Finally, Arhgdib, the tumor suppressor gene whose expression is associated with GM3, was shown to be upstream of TNF-α. Conclusions: The GM3 signal is thus transduced in B16 cells through a PI3K, Rictor/mTOR, Akt, Arhgdib pathway, leading to stimulated expression ofTNF-α.

Original languageEnglish
Pages (from-to)430-438
Number of pages9
JournalOncology
Volume73
Issue number5-6
DOIs
Publication statusPublished - 2008 Jun

Fingerprint

Guanine Nucleotide Dissociation Inhibitors
Experimental Melanomas
Phosphatidylinositol 3-Kinases
Tumor Necrosis Factor-alpha
Raptors
Small Interfering RNA
LY 303511
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Sirolimus
Tumor Suppressor Genes
Phosphorylation
Gene Expression

Keywords

  • Arhgdib
  • Ganglioside
  • GM3
  • MTOR
  • Raptor
  • Rictor
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

GM3 signals regulating TNF-alpha expression are mediated by rictor and Arhgdib in mouse melanoma B16 cells. / Wang, Pu; Yang, Xiaoyan; Wu, Peixing; Zhang, Jinghai; Sato, Toshinori; Yamagata, Sadako; Yamagata, Tatsuya.

In: Oncology, Vol. 73, No. 5-6, 06.2008, p. 430-438.

Research output: Contribution to journalArticle

Wang, P, Yang, X, Wu, P, Zhang, J, Sato, T, Yamagata, S & Yamagata, T 2008, 'GM3 signals regulating TNF-alpha expression are mediated by rictor and Arhgdib in mouse melanoma B16 cells', Oncology, vol. 73, no. 5-6, pp. 430-438. https://doi.org/10.1159/000136801
Wang, Pu ; Yang, Xiaoyan ; Wu, Peixing ; Zhang, Jinghai ; Sato, Toshinori ; Yamagata, Sadako ; Yamagata, Tatsuya. / GM3 signals regulating TNF-alpha expression are mediated by rictor and Arhgdib in mouse melanoma B16 cells. In: Oncology. 2008 ; Vol. 73, No. 5-6. pp. 430-438.
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T1 - GM3 signals regulating TNF-alpha expression are mediated by rictor and Arhgdib in mouse melanoma B16 cells

AU - Wang, Pu

AU - Yang, Xiaoyan

AU - Wu, Peixing

AU - Zhang, Jinghai

AU - Sato, Toshinori

AU - Yamagata, Sadako

AU - Yamagata, Tatsuya

PY - 2008/6

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N2 - Objective: We have previously shown GM3 to positively regulate TNF-α expression via a PI3K/Akt pathway in mouse melanoma B16 cells [Wang et al.: Biochem Biophys Res Commun 2007;356:438-443]. The GM3 signal was shown to be located upstream of Akt, but whether it is located upstream of PI3K and which molecule is the effector of PI3K remain to be clarified. Methods: We used inhibitors of PI3K and mTOR, and siRNA directed to Rictor, Raptor and Rho-GDP dissociation inhibitor β (Arhgdib). Results: PI3K inhibitors LY294002 and LY303511 were shown to suppress TNF-α expression that is stimulated by GM3 in B16 cells, suggesting that the GM3 signal is located upstream of the PI3K-Akt pathway. Rapamycin suppressed TNF-α expression, indicating mTOR to be involved in the pathway. Either siRNA Raptor or siRNA Rictor suppressed TNF-α expression, but the latter suppressed the effects of GM3 on TNF-α expression and Akt phosphorylation at Ser473, indicating the GM3 signal to be transduced via Rictor/mTOR and Akt (Ser473), leading to TNF-α stimulation. Finally, Arhgdib, the tumor suppressor gene whose expression is associated with GM3, was shown to be upstream of TNF-α. Conclusions: The GM3 signal is thus transduced in B16 cells through a PI3K, Rictor/mTOR, Akt, Arhgdib pathway, leading to stimulated expression ofTNF-α.

AB - Objective: We have previously shown GM3 to positively regulate TNF-α expression via a PI3K/Akt pathway in mouse melanoma B16 cells [Wang et al.: Biochem Biophys Res Commun 2007;356:438-443]. The GM3 signal was shown to be located upstream of Akt, but whether it is located upstream of PI3K and which molecule is the effector of PI3K remain to be clarified. Methods: We used inhibitors of PI3K and mTOR, and siRNA directed to Rictor, Raptor and Rho-GDP dissociation inhibitor β (Arhgdib). Results: PI3K inhibitors LY294002 and LY303511 were shown to suppress TNF-α expression that is stimulated by GM3 in B16 cells, suggesting that the GM3 signal is located upstream of the PI3K-Akt pathway. Rapamycin suppressed TNF-α expression, indicating mTOR to be involved in the pathway. Either siRNA Raptor or siRNA Rictor suppressed TNF-α expression, but the latter suppressed the effects of GM3 on TNF-α expression and Akt phosphorylation at Ser473, indicating the GM3 signal to be transduced via Rictor/mTOR and Akt (Ser473), leading to TNF-α stimulation. Finally, Arhgdib, the tumor suppressor gene whose expression is associated with GM3, was shown to be upstream of TNF-α. Conclusions: The GM3 signal is thus transduced in B16 cells through a PI3K, Rictor/mTOR, Akt, Arhgdib pathway, leading to stimulated expression ofTNF-α.

KW - Arhgdib

KW - Ganglioside

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KW - MTOR

KW - Raptor

KW - Rictor

KW - Tumor necrosis factor-α

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