Abstract
We used the Gorog Thrombosis Test to analyze the factors influencing the occlusion time, which represents platelet activation and subsequent occlusive thrombus formation, in 132 healthy Japanese volunteers (116 men, 16 women; mean age, 45.0 ± 12.0 years). The Gorog Thrombosis Test was designed to evaluate platelet aggregation and thrombolytic activity under a high shear stress condition (175 dynes/cm2) in a native blood sample in vitro. The mean ± SD occlusion time was 154.8 ± 64.7 s (men, 153.4 ± 64.2 s and women, 165.4 ± 56.5 s). The occlusion time was inversely correlated with von Willebrand factor ristocetin cofactor activity (VWF:Rco) (r = -0.242, P = 0.0055) and von Willebrand factor antigen (r = -0.230, P = 0.0080). The mean occlusion time in the group with VWF:Rco of at least 170% (137 s) was significantly shorter than that in the group with VWF:Rco less than 170% (156 s, P < 0.05). Platelet counts, other coagulation markers and smoking showed no significant correlations with occlusion time. Red blood cells (r = -0.177, P = 0.0365), hemoglobin (r = -0.191, P = 0.0245) and hematocrit (r = -0.182, P = 0.0329) also showed inverse correlations with the occlusion time. This report is the first to clearly demonstrate the role of von Willebrand factor in the formation of occlusive thrombi in the Gorog Thrombosis Test.
| Original language | English |
|---|---|
| Pages (from-to) | 203-207 |
| Number of pages | 5 |
| Journal | Blood Coagulation and Fibrinolysis |
| Volume | 17 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2006 Apr |
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Keywords
- Gorog Thrombosis Test
- Hematocrit
- Hemoglobin
- Occlusion time
- Red blood cells
- Thrombus formation
- Von Willebrand factor antigen
- Von willebrand factor ristocetin cofactor activity
ASJC Scopus subject areas
- Hematology
Cite this
Gorog thrombosis test : Analysis of factors influencing occlusive thrombus formation. / Nishida, Hiroko; Murata, Mitsuru; Miyaki, Koichi; Omae, Kazuyuki; Watanabe, Kiyoaki; Ikeda, Yasuo.
In: Blood Coagulation and Fibrinolysis, Vol. 17, No. 3, 04.2006, p. 203-207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gorog thrombosis test
T2 - Analysis of factors influencing occlusive thrombus formation
AU - Nishida, Hiroko
AU - Murata, Mitsuru
AU - Miyaki, Koichi
AU - Omae, Kazuyuki
AU - Watanabe, Kiyoaki
AU - Ikeda, Yasuo
PY - 2006/4
Y1 - 2006/4
N2 - We used the Gorog Thrombosis Test to analyze the factors influencing the occlusion time, which represents platelet activation and subsequent occlusive thrombus formation, in 132 healthy Japanese volunteers (116 men, 16 women; mean age, 45.0 ± 12.0 years). The Gorog Thrombosis Test was designed to evaluate platelet aggregation and thrombolytic activity under a high shear stress condition (175 dynes/cm2) in a native blood sample in vitro. The mean ± SD occlusion time was 154.8 ± 64.7 s (men, 153.4 ± 64.2 s and women, 165.4 ± 56.5 s). The occlusion time was inversely correlated with von Willebrand factor ristocetin cofactor activity (VWF:Rco) (r = -0.242, P = 0.0055) and von Willebrand factor antigen (r = -0.230, P = 0.0080). The mean occlusion time in the group with VWF:Rco of at least 170% (137 s) was significantly shorter than that in the group with VWF:Rco less than 170% (156 s, P < 0.05). Platelet counts, other coagulation markers and smoking showed no significant correlations with occlusion time. Red blood cells (r = -0.177, P = 0.0365), hemoglobin (r = -0.191, P = 0.0245) and hematocrit (r = -0.182, P = 0.0329) also showed inverse correlations with the occlusion time. This report is the first to clearly demonstrate the role of von Willebrand factor in the formation of occlusive thrombi in the Gorog Thrombosis Test.
AB - We used the Gorog Thrombosis Test to analyze the factors influencing the occlusion time, which represents platelet activation and subsequent occlusive thrombus formation, in 132 healthy Japanese volunteers (116 men, 16 women; mean age, 45.0 ± 12.0 years). The Gorog Thrombosis Test was designed to evaluate platelet aggregation and thrombolytic activity under a high shear stress condition (175 dynes/cm2) in a native blood sample in vitro. The mean ± SD occlusion time was 154.8 ± 64.7 s (men, 153.4 ± 64.2 s and women, 165.4 ± 56.5 s). The occlusion time was inversely correlated with von Willebrand factor ristocetin cofactor activity (VWF:Rco) (r = -0.242, P = 0.0055) and von Willebrand factor antigen (r = -0.230, P = 0.0080). The mean occlusion time in the group with VWF:Rco of at least 170% (137 s) was significantly shorter than that in the group with VWF:Rco less than 170% (156 s, P < 0.05). Platelet counts, other coagulation markers and smoking showed no significant correlations with occlusion time. Red blood cells (r = -0.177, P = 0.0365), hemoglobin (r = -0.191, P = 0.0245) and hematocrit (r = -0.182, P = 0.0329) also showed inverse correlations with the occlusion time. This report is the first to clearly demonstrate the role of von Willebrand factor in the formation of occlusive thrombi in the Gorog Thrombosis Test.
KW - Gorog Thrombosis Test
KW - Hematocrit
KW - Hemoglobin
KW - Occlusion time
KW - Red blood cells
KW - Thrombus formation
KW - Von Willebrand factor antigen
KW - Von willebrand factor ristocetin cofactor activity
UR - http://www.scopus.com/inward/record.url?scp=33645517506&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645517506&partnerID=8YFLogxK
U2 - 10.1007/s10577-006-1036-7
DO - 10.1007/s10577-006-1036-7
M3 - Article
C2 - 16575258
AN - SCOPUS:33645517506
VL - 17
SP - 203
EP - 207
JO - Blood Coagulation and Fibrinolysis
JF - Blood Coagulation and Fibrinolysis
SN - 0957-5235
IS - 3
ER -