Grafted swine neuroepithelial stem cells can form myelinated axons and both efferent and afferent synapses with xenogeneic rat neurons

Koichi Uchida, Hideyuki Okano, Takuro Hayashi, Yutaka Mine, Yoshikuni Tanioka, Tatsuji Nomura, Takeshi Kawase

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Neuroepithelial stem cells derived from the swine mesencephalic neural tube were examined regarding their eligibility for neural xenografting as a donor material, with the aim of evaluating myelinated axon formation and both types of synaptic formation with xenogeneic host neurons as part of possible neural circuit reconstruction. The mesencephalic neural tube tissues were dissected out from swine embryos at embryonic days 17 and 18 and were implanted immediately into the striatum of the Parkinsonian model rat. The swine-derived grafts had many nestin-positive rosette-forming, neurofilament-positive, and tyrosine hydroxylase-positive cells in the rat striatum. Electron microscopic study revealed both efferent and afferent synaptic formations in the donor-derived immature neurons or tyrosine hydroxylase-positive donor cells in the grafts. Myelinated axons, both positive and negative for swine-specific neurofilament antibody, were mingled together in the graft. These results indicated that implanted neuroepithelial stem cells could survive well and divide asymmetrically into both nestin-expressing precursors and differentiated neurochemical marker-expressing neurons in the xenogeneic rat striatum, with the help of an immunosuppressant. Donor-derived immature neurons formed both efferent and afferent synapses with xenogeneic host neurons, and donor-derived axons were myelinated, which suggests that implanted swine neuroepithelial stem cells could possibly restore damaged neuronal circuitry in the diseased brain.

Original languageEnglish
Pages (from-to)661-669
Number of pages9
JournalJournal of Neuroscience Research
Volume72
Issue number6
DOIs
Publication statusPublished - 2003 Jun 15

Fingerprint

Neuroepithelial Cells
Synapses
Axons
Swine
Stem Cells
Neurons
Nestin
Neural Tube
Intermediate Filaments
Tyrosine 3-Monooxygenase
Transplants
Heterologous Transplantation
Brain Diseases
Immunosuppressive Agents
Embryonic Structures
Electrons
Antibodies

Keywords

  • Myelin
  • Neural xenograft
  • Neuroepithelial stem cell
  • Swine
  • Synapse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Grafted swine neuroepithelial stem cells can form myelinated axons and both efferent and afferent synapses with xenogeneic rat neurons. / Uchida, Koichi; Okano, Hideyuki; Hayashi, Takuro; Mine, Yutaka; Tanioka, Yoshikuni; Nomura, Tatsuji; Kawase, Takeshi.

In: Journal of Neuroscience Research, Vol. 72, No. 6, 15.06.2003, p. 661-669.

Research output: Contribution to journalArticle

Uchida, Koichi ; Okano, Hideyuki ; Hayashi, Takuro ; Mine, Yutaka ; Tanioka, Yoshikuni ; Nomura, Tatsuji ; Kawase, Takeshi. / Grafted swine neuroepithelial stem cells can form myelinated axons and both efferent and afferent synapses with xenogeneic rat neurons. In: Journal of Neuroscience Research. 2003 ; Vol. 72, No. 6. pp. 661-669.
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