Granulocyte and monocyte adsorption apheresis therapy modulates monocyte-derived dendritic cell function in patients with ulcerative colitis

Yuko Iwakami, Atsushi Sakuraba, Toshiro Sato, Yasuhiro Takada, Motoko Izumiya, Hitoshi Ichikawa, Toshifumi Hibi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The aim of this study was to elucidate the molecular mechanisms responsible for the therapeutic effects of granulocyte and monocyte adsorption apheresis (GMA). We investigated the alterations in circulating monocyte subsets and monocyte-derived dendritic cell (moDC) function after GMA therapy in ulcerative colitis (UC) patients. Eighteen patients with UC were enrolled: 14 patients were responders, and 4 patients were non-responders. Peripheral venous blood was obtained within 5 min before and 5 min after GMA therapy. Flow cytometric analysis for monocyte markers (CD14/CD16) was then performed. Monocyte-derived dendritic cells were obtained and alterations in their phenotype were analyzed by flow cytometry. Their function was also analyzed in a mixed lymphocyte reaction assay between allo-naïve T lymphocytes. Flow cytometric analysis for intracellular interferon (IFN)-γ (T-helper 1 cells) and interleukin (IL)-4 (T-helper 2 cells) was then performed for the stimulated T lymphocytes. In patients who responded to GMA, the average numbers of monocytes, especially CD16+ monocytes, were significantly decreased after therapy (P < 0.05). In responders, post-GMA moDCs expressed significantly lower CD80 and B7-DC, which are one of the stimulation and maturation markers of dendritic cells, compared to pre-GMA moDCs. CD83, CD86 and human leukocyte antigen-DR also showed a tendency to decrease. In responders, naïve T lymphocytes stimulated with post-GMA moDCs produced significantly less IFN-γ and IL-4 compared to those stimulated with pre-GMA moDCs. The results of our study show that some of the immunosuppressive effects of GMA therapy may be associated with the modulation of monocyte subsets and moDC function.

Original languageEnglish
Pages (from-to)138-146
Number of pages9
JournalTherapeutic Apheresis and Dialysis
Volume13
Issue number2
DOIs
Publication statusPublished - 2009 Apr

Fingerprint

Blood Component Removal
Ulcerative Colitis
Granulocytes
Dendritic Cells
Adsorption
Monocytes
Therapeutics
T-Lymphocytes
Interleukin-4
Interferons
Th2 Cells
Th1 Cells
Mixed Lymphocyte Culture Test
Therapeutic Uses
Immunosuppressive Agents

Keywords

  • Dendritic cell
  • Granulocyte and monocyte adsorption apheresis
  • Ulcerative colitis

ASJC Scopus subject areas

  • Hematology
  • Nephrology

Cite this

Granulocyte and monocyte adsorption apheresis therapy modulates monocyte-derived dendritic cell function in patients with ulcerative colitis. / Iwakami, Yuko; Sakuraba, Atsushi; Sato, Toshiro; Takada, Yasuhiro; Izumiya, Motoko; Ichikawa, Hitoshi; Hibi, Toshifumi.

In: Therapeutic Apheresis and Dialysis, Vol. 13, No. 2, 04.2009, p. 138-146.

Research output: Contribution to journalArticle

Iwakami, Yuko ; Sakuraba, Atsushi ; Sato, Toshiro ; Takada, Yasuhiro ; Izumiya, Motoko ; Ichikawa, Hitoshi ; Hibi, Toshifumi. / Granulocyte and monocyte adsorption apheresis therapy modulates monocyte-derived dendritic cell function in patients with ulcerative colitis. In: Therapeutic Apheresis and Dialysis. 2009 ; Vol. 13, No. 2. pp. 138-146.
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