Granulocyte colony stimulating factor-producing multiple myeloma associated with neutrophilia

Kanoka Kohmura, Yoshitaka Miyakawa, Kaori Kameyama, Masahiro Kizaki, Yasuo Ikeda

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

We report a case of IgG-κ multiple myeloma associated with neutrophilia (WBC 31,300/μl, neutrophil 90.5%). Interestingly, the serum level of granulocyte colony stimulating factor (G-CSF) in this patient was elevated to 1,500 pg/ml (normal range: 5.78-27.5). Plasma cells were 35% in the bone marrow and were strongly stained with anti-G-CSF antibody. To directly study the production of G-CSF from plasma cells in this patient, CD138 positive plasma cells were purified from bone marrow of multiple myeloma patients by magnetic sorting. The expression of G-CSF mRNA was observed in CD138 positive plasma cells from this myeloma patient with neutrophilia by RT-PCR. In contrast, the expression of G-CSF mRNA was not detected in CD138 positive plasma cells from the other multiple myeloma patients without neutrophilia and 4 human myeloma cell lines (HS-Sultan, IM9, RPMI8226, U266) by RT-PCR. After the CD138 positive plasma cells were cultured in vitro for 48 h, the production of G-CSF protein was confirmed (71.8 pg/ml) in the supernatant by ELISA. These results indicated plasma cells of this myeloma patient directly produced G-CSF and that this was the primary cause of neutrophilia.

Original languageEnglish
Pages (from-to)1475-1479
Number of pages5
JournalLeukemia and Lymphoma
Volume45
Issue number7
DOIs
Publication statusPublished - 2004 Jul 1

Keywords

  • G-CSF
  • Multiple myeloma
  • Neutrophilia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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