Granulocyte colony stimulating factor-producing multiple myeloma associated with neutrophilia

Kanoka Kohmura, Yoshitaka Miyakawa, Kaori Kameyama, Masahiro Kizaki, Yasuo Ikeda

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We report a case of IgG-κ multiple myeloma associated with neutrophilia (WBC 31,300/μl, neutrophil 90.5%). Interestingly, the serum level of granulocyte colony stimulating factor (G-CSF) in this patient was elevated to 1,500 pg/ml (normal range: 5.78-27.5). Plasma cells were 35% in the bone marrow and were strongly stained with anti-G-CSF antibody. To directly study the production of G-CSF from plasma cells in this patient, CD138 positive plasma cells were purified from bone marrow of multiple myeloma patients by magnetic sorting. The expression of G-CSF mRNA was observed in CD138 positive plasma cells from this myeloma patient with neutrophilia by RT-PCR. In contrast, the expression of G-CSF mRNA was not detected in CD138 positive plasma cells from the other multiple myeloma patients without neutrophilia and 4 human myeloma cell lines (HS-Sultan, IM9, RPMI8226, U266) by RT-PCR. After the CD138 positive plasma cells were cultured in vitro for 48 h, the production of G-CSF protein was confirmed (71.8 pg/ml) in the supernatant by ELISA. These results indicated plasma cells of this myeloma patient directly produced G-CSF and that this was the primary cause of neutrophilia.

Original languageEnglish
Pages (from-to)1475-1479
Number of pages5
JournalLeukemia and Lymphoma
Volume45
Issue number7
DOIs
Publication statusPublished - 2004 Jul

Fingerprint

Granulocyte Colony-Stimulating Factor
Multiple Myeloma
Plasma Cells
Bone Marrow
Polymerase Chain Reaction
Messenger RNA
Reference Values
Neutrophils
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Cell Line
Antibodies
Serum
Proteins

Keywords

  • G-CSF
  • Multiple myeloma
  • Neutrophilia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Granulocyte colony stimulating factor-producing multiple myeloma associated with neutrophilia. / Kohmura, Kanoka; Miyakawa, Yoshitaka; Kameyama, Kaori; Kizaki, Masahiro; Ikeda, Yasuo.

In: Leukemia and Lymphoma, Vol. 45, No. 7, 07.2004, p. 1475-1479.

Research output: Contribution to journalArticle

Kohmura, Kanoka ; Miyakawa, Yoshitaka ; Kameyama, Kaori ; Kizaki, Masahiro ; Ikeda, Yasuo. / Granulocyte colony stimulating factor-producing multiple myeloma associated with neutrophilia. In: Leukemia and Lymphoma. 2004 ; Vol. 45, No. 7. pp. 1475-1479.
@article{e86f5a299f1e48b9a00bb0a2560d49aa,
title = "Granulocyte colony stimulating factor-producing multiple myeloma associated with neutrophilia",
abstract = "We report a case of IgG-κ multiple myeloma associated with neutrophilia (WBC 31,300/μl, neutrophil 90.5{\%}). Interestingly, the serum level of granulocyte colony stimulating factor (G-CSF) in this patient was elevated to 1,500 pg/ml (normal range: 5.78-27.5). Plasma cells were 35{\%} in the bone marrow and were strongly stained with anti-G-CSF antibody. To directly study the production of G-CSF from plasma cells in this patient, CD138 positive plasma cells were purified from bone marrow of multiple myeloma patients by magnetic sorting. The expression of G-CSF mRNA was observed in CD138 positive plasma cells from this myeloma patient with neutrophilia by RT-PCR. In contrast, the expression of G-CSF mRNA was not detected in CD138 positive plasma cells from the other multiple myeloma patients without neutrophilia and 4 human myeloma cell lines (HS-Sultan, IM9, RPMI8226, U266) by RT-PCR. After the CD138 positive plasma cells were cultured in vitro for 48 h, the production of G-CSF protein was confirmed (71.8 pg/ml) in the supernatant by ELISA. These results indicated plasma cells of this myeloma patient directly produced G-CSF and that this was the primary cause of neutrophilia.",
keywords = "G-CSF, Multiple myeloma, Neutrophilia",
author = "Kanoka Kohmura and Yoshitaka Miyakawa and Kaori Kameyama and Masahiro Kizaki and Yasuo Ikeda",
year = "2004",
month = "7",
doi = "10.1080/10428190310001645870",
language = "English",
volume = "45",
pages = "1475--1479",
journal = "Leukemia and Lymphoma",
issn = "1042-8194",
publisher = "Informa Healthcare",
number = "7",

}

TY - JOUR

T1 - Granulocyte colony stimulating factor-producing multiple myeloma associated with neutrophilia

AU - Kohmura, Kanoka

AU - Miyakawa, Yoshitaka

AU - Kameyama, Kaori

AU - Kizaki, Masahiro

AU - Ikeda, Yasuo

PY - 2004/7

Y1 - 2004/7

N2 - We report a case of IgG-κ multiple myeloma associated with neutrophilia (WBC 31,300/μl, neutrophil 90.5%). Interestingly, the serum level of granulocyte colony stimulating factor (G-CSF) in this patient was elevated to 1,500 pg/ml (normal range: 5.78-27.5). Plasma cells were 35% in the bone marrow and were strongly stained with anti-G-CSF antibody. To directly study the production of G-CSF from plasma cells in this patient, CD138 positive plasma cells were purified from bone marrow of multiple myeloma patients by magnetic sorting. The expression of G-CSF mRNA was observed in CD138 positive plasma cells from this myeloma patient with neutrophilia by RT-PCR. In contrast, the expression of G-CSF mRNA was not detected in CD138 positive plasma cells from the other multiple myeloma patients without neutrophilia and 4 human myeloma cell lines (HS-Sultan, IM9, RPMI8226, U266) by RT-PCR. After the CD138 positive plasma cells were cultured in vitro for 48 h, the production of G-CSF protein was confirmed (71.8 pg/ml) in the supernatant by ELISA. These results indicated plasma cells of this myeloma patient directly produced G-CSF and that this was the primary cause of neutrophilia.

AB - We report a case of IgG-κ multiple myeloma associated with neutrophilia (WBC 31,300/μl, neutrophil 90.5%). Interestingly, the serum level of granulocyte colony stimulating factor (G-CSF) in this patient was elevated to 1,500 pg/ml (normal range: 5.78-27.5). Plasma cells were 35% in the bone marrow and were strongly stained with anti-G-CSF antibody. To directly study the production of G-CSF from plasma cells in this patient, CD138 positive plasma cells were purified from bone marrow of multiple myeloma patients by magnetic sorting. The expression of G-CSF mRNA was observed in CD138 positive plasma cells from this myeloma patient with neutrophilia by RT-PCR. In contrast, the expression of G-CSF mRNA was not detected in CD138 positive plasma cells from the other multiple myeloma patients without neutrophilia and 4 human myeloma cell lines (HS-Sultan, IM9, RPMI8226, U266) by RT-PCR. After the CD138 positive plasma cells were cultured in vitro for 48 h, the production of G-CSF protein was confirmed (71.8 pg/ml) in the supernatant by ELISA. These results indicated plasma cells of this myeloma patient directly produced G-CSF and that this was the primary cause of neutrophilia.

KW - G-CSF

KW - Multiple myeloma

KW - Neutrophilia

UR - http://www.scopus.com/inward/record.url?scp=2542513064&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542513064&partnerID=8YFLogxK

U2 - 10.1080/10428190310001645870

DO - 10.1080/10428190310001645870

M3 - Article

C2 - 15359652

AN - SCOPUS:2542513064

VL - 45

SP - 1475

EP - 1479

JO - Leukemia and Lymphoma

JF - Leukemia and Lymphoma

SN - 1042-8194

IS - 7

ER -