Green marker for colonies of Bacillus subtilis

Mitsuhiro Itaya, Syed M. Shaheduzzaman, Kuniko Matsui, Akira Omori, Takashi Tsuji

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.

Original languageEnglish
Pages (from-to)579-583
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number3
DOIs
Publication statusPublished - 2001 Mar
Externally publishedYes

Fingerprint

Bacilli
Bacillus subtilis
plasmids
Plasmids
Fluorescence
fluorescence
translation (genetics)
blasticidin S
Genes
Antibiotics
Ultraviolet Rays
Ultraviolet radiation
Agar
ultraviolet radiation
genes
Cell Count
agar
antibiotics
promoter regions
Anti-Bacterial Agents

Keywords

  • Bacillus subtilis
  • Fused protein
  • Green fluorescence protein
  • Multi-copy plasmid

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Itaya, M., Shaheduzzaman, S. M., Matsui, K., Omori, A., & Tsuji, T. (2001). Green marker for colonies of Bacillus subtilis. Bioscience, Biotechnology and Biochemistry, 65(3), 579-583. https://doi.org/10.1271/bbb.65.579

Green marker for colonies of Bacillus subtilis. / Itaya, Mitsuhiro; Shaheduzzaman, Syed M.; Matsui, Kuniko; Omori, Akira; Tsuji, Takashi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 3, 03.2001, p. 579-583.

Research output: Contribution to journalArticle

Itaya, M, Shaheduzzaman, SM, Matsui, K, Omori, A & Tsuji, T 2001, 'Green marker for colonies of Bacillus subtilis', Bioscience, Biotechnology and Biochemistry, vol. 65, no. 3, pp. 579-583. https://doi.org/10.1271/bbb.65.579
Itaya M, Shaheduzzaman SM, Matsui K, Omori A, Tsuji T. Green marker for colonies of Bacillus subtilis. Bioscience, Biotechnology and Biochemistry. 2001 Mar;65(3):579-583. https://doi.org/10.1271/bbb.65.579
Itaya, Mitsuhiro ; Shaheduzzaman, Syed M. ; Matsui, Kuniko ; Omori, Akira ; Tsuji, Takashi. / Green marker for colonies of Bacillus subtilis. In: Bioscience, Biotechnology and Biochemistry. 2001 ; Vol. 65, No. 3. pp. 579-583.
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