@article{0c48743b884242c78d772459e945af7d,
title = "Growth Cone Phosphoproteomics Reveals that GAP-43 Phosphorylated by JNK Is a Marker of Axon Growth and Regeneration",
abstract = "Neuroscience; Developmental Neuroscience; Bioinformatics; Proteomics",
keywords = "Bioinformatics, Developmental Neuroscience, Neuroscience, Proteomics",
author = "Asami Kawasaki and Masayasu Okada and Atsushi Tamada and Shujiro Okuda and Motohiro Nozumi and Yasuyuki Ito and Daiki Kobayashi and Tokiwa Yamasaki and Ryo Yokoyama and Takeshi Shibata and Hiroshi Nishina and Yutaka Yoshida and Yukihiko Fujii and Kosei Takeuchi and Michihiro Igarashi",
note = "Funding Information: We thank T. Tsubata (ABSciex Co. Ltd.)and M. Matsumoto (Kyushu University, Fukuoka, Japan)for discussions concerning phosphoproteomics, Profs. T. Kanki, M. Komatsu, and H. Takebayashi (Niigata University, Japan)for their comments, and A. Ikarashi (Igarashi laboratory)for excellent technical assistance with the proteomics studies. This work was supported in part by KAKENHI from JSPS and MEXT of Japan (#17023019, #221S0003, #22240040, #24111515, #18H04670, and #18H04013 to M.I.; #24110503, #26462232, and #26110703 to K.T.; #23111532, #24650163, #15K14522, and #16H01423 to A.T.; #17K17739 to M.O.; #24700318 and #18K06459 to M.N.; #25870251 to A.K.; #26700029 to S.O.; and #25461212 to Y.Y.); by JST-Presto (to A.T.); and by grants from Yujin Memorial Foundation (to K.T. and M.N.), Kyowa-kai Foundation (to M.O. and D.K.), Takeda Science Foundation (to M.N. and Y.I.), Uehara Memorial Foundation for Life Sciences (to M.I. and Y.I.), and the Project-Promoting Grant from Niigata University (to M.I.). This paper is dedicated as a memorial to the late Karl H. Pfenninger (1944–2015; University of Colorado, Denver, CO, USA), who first established the method for the isolation of growth cones in 1983. M.I. conceived the study. A.K. and D.K. performed the biochemical and pharmacological analysis of GAP-43 phosphorylation. M.I. Y.Y. Y.I. and M.O. analyzed the phosphoproteomics of the sciatic nerve. M.I. M.N. T.S. and R.Y. performed the GCM phosphoproteomics. M.I. K.T. and A.K. established and characterized the phospho-specific antibodies. A.T. analyzed immunohistochemistry during development. S.O. A.T. and M.I. performed the bioinformatics analysis. M.I. Y.F. and M.O. designed the nerve regeneration study, and M.O. performed it. H.N. and T.Y. produced and provided the MKK7-cKO mice. M.I. wrote the whole of the manuscript, and M.I. A.K. M.O. A.T. S.O. and Y.Y. wrote the Transparent Methods (see Supplemental Information)and the legends of the figures. R.Y. and T.S. are employees of Sciex Japan. K.T. M.I. M.N. and A.K. have a patent EP2769988 (anti-GAP-43 antibody, date of publication of application: 27 August, 2014), which is related to this work, but there are no patent royalties and licensing fees. Other authors declare no COI. Funding Information: We thank T. Tsubata (ABSciex Co. Ltd.) and M. Matsumoto (Kyushu University, Fukuoka, Japan) for discussions concerning phosphoproteomics, Profs. T. Kanki, M. Komatsu, and H. Takebayashi (Niigata University, Japan) for their comments, and A. Ikarashi (Igarashi laboratory) for excellent technical assistance with the proteomics studies. This work was supported in part by KAKENHI from JSPS and MEXT of Japan (# 17023019 , # 221S0003 , # 22240040 , # 24111515 , # 18H04670 , and # 18H04013 to M.I.; # 24110503 , # 26462232 , and # 26110703 to K.T.; # 23111532 , # 24650163 , # 15K14522 , and # 16H01423 to A.T.; # 17K17739 to M.O.; # 24700318 and # 18K06459 to M.N.; # 25870251 to A.K.; # 26700029 to S.O.; and # 25461212 to Y.Y.); by JST -Presto (to A.T.); and by grants from Yujin Memorial Foundation (to K.T. and M.N.), Kyowa-kai Foundation (to M.O. and D.K.), Takeda Science Foundation (to M.N. and Y.I.), Uehara Memorial Foundation for Life Sciences (to M.I. and Y.I.), and the Project-Promoting Grant from Niigata University (to M.I.). This paper is dedicated as a memorial to the late Karl H. Pfenninger (1944–2015; University of Colorado, Denver, CO, USA), who first established the method for the isolation of growth cones in 1983. Publisher Copyright: {\textcopyright} 2018 The Authors",
year = "2018",
month = jun,
day = "29",
doi = "10.1016/j.isci.2018.05.019",
language = "English",
volume = "4",
pages = "190--203",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
}