Growth factors influence contractility and α-smooth muscle actin expression in bovine lens epithelial cells

D. Kurosaka, K. Kato, T. Nagamoto, Kazuno Negishi

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Purpose. To determine whether basic fibroblast growth factor (bFGF) and transforming growth factor-β2 (TGF-β2) influence the contractile activity and the expression of α-smooth muscle actin (α-SMA) in bovine lens epithelial cells (LECs). To examine whether modulation of contractile activity by these growth factors depends on changes of α-SMA expression. Methods. Bovine LECs were cultured in collagen gel in MED 5 medium (F-12 nutrient mixture supplemented with 5% fatal bovine scrimp) with or without bFGF (1 to 100 ng/ml) or TGF-β2 (0.01 to 10 ng/ml). To evaluate collagen gel contraction, the longest and shortest diameters of the gels were measured daily for 7 days, and the area was determined. Detection of α-SMA in the gels was performed immunohistochemically using a mouse monoclonal antibody against α-SMA. The percentage of α-SMA-positive cells to the total number of cells was determined. Results. Control gels cultured with MED 5 medium alone contracted to 15.8% ± 3.4% of their original area after 7 days. TGF- β2 significantly increased this contraction in a dose-dependent manner, whereas bFGF significantly decreased it. Approximately 30% of cells in the control gels were α-SMA positive. TGF-β2 significantly increased the α- SMA positivity dose dependently, whereas bFGF significantly decreased it. The percent positivity for α-SMA and the gel area showed a significant negative correlation. Conclusions. TGF-β2 increased collagen gel contraction and α- SMA expression in bovine LECs, whereas bFGF decreased these parameters. Because collagen gel contraction was correlated with α-SMA expression, the modulation of LEC contractile activity by growth factors may be related to an effect on α-SMA.

Original languageEnglish
Pages (from-to)1701-1708
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume36
Issue number8
Publication statusPublished - 1995

Fingerprint

Lenses
Smooth Muscle
Actins
Intercellular Signaling Peptides and Proteins
Gels
Epithelial Cells
Fibroblast Growth Factor 2
Transforming Growth Factors
Collagen
Fibroblast Growth Factor 1
Cell Count
Monoclonal Antibodies
Food

Keywords

  • alpha-smooth muscle actin
  • basic fibroblast growth factor
  • collagen gel
  • lens epithelial cell
  • transforming growth factor-beta

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Growth factors influence contractility and α-smooth muscle actin expression in bovine lens epithelial cells. / Kurosaka, D.; Kato, K.; Nagamoto, T.; Negishi, Kazuno.

In: Investigative Ophthalmology and Visual Science, Vol. 36, No. 8, 1995, p. 1701-1708.

Research output: Contribution to journalArticle

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title = "Growth factors influence contractility and α-smooth muscle actin expression in bovine lens epithelial cells",
abstract = "Purpose. To determine whether basic fibroblast growth factor (bFGF) and transforming growth factor-β2 (TGF-β2) influence the contractile activity and the expression of α-smooth muscle actin (α-SMA) in bovine lens epithelial cells (LECs). To examine whether modulation of contractile activity by these growth factors depends on changes of α-SMA expression. Methods. Bovine LECs were cultured in collagen gel in MED 5 medium (F-12 nutrient mixture supplemented with 5{\%} fatal bovine scrimp) with or without bFGF (1 to 100 ng/ml) or TGF-β2 (0.01 to 10 ng/ml). To evaluate collagen gel contraction, the longest and shortest diameters of the gels were measured daily for 7 days, and the area was determined. Detection of α-SMA in the gels was performed immunohistochemically using a mouse monoclonal antibody against α-SMA. The percentage of α-SMA-positive cells to the total number of cells was determined. Results. Control gels cultured with MED 5 medium alone contracted to 15.8{\%} ± 3.4{\%} of their original area after 7 days. TGF- β2 significantly increased this contraction in a dose-dependent manner, whereas bFGF significantly decreased it. Approximately 30{\%} of cells in the control gels were α-SMA positive. TGF-β2 significantly increased the α- SMA positivity dose dependently, whereas bFGF significantly decreased it. The percent positivity for α-SMA and the gel area showed a significant negative correlation. Conclusions. TGF-β2 increased collagen gel contraction and α- SMA expression in bovine LECs, whereas bFGF decreased these parameters. Because collagen gel contraction was correlated with α-SMA expression, the modulation of LEC contractile activity by growth factors may be related to an effect on α-SMA.",
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T1 - Growth factors influence contractility and α-smooth muscle actin expression in bovine lens epithelial cells

AU - Kurosaka, D.

AU - Kato, K.

AU - Nagamoto, T.

AU - Negishi, Kazuno

PY - 1995

Y1 - 1995

N2 - Purpose. To determine whether basic fibroblast growth factor (bFGF) and transforming growth factor-β2 (TGF-β2) influence the contractile activity and the expression of α-smooth muscle actin (α-SMA) in bovine lens epithelial cells (LECs). To examine whether modulation of contractile activity by these growth factors depends on changes of α-SMA expression. Methods. Bovine LECs were cultured in collagen gel in MED 5 medium (F-12 nutrient mixture supplemented with 5% fatal bovine scrimp) with or without bFGF (1 to 100 ng/ml) or TGF-β2 (0.01 to 10 ng/ml). To evaluate collagen gel contraction, the longest and shortest diameters of the gels were measured daily for 7 days, and the area was determined. Detection of α-SMA in the gels was performed immunohistochemically using a mouse monoclonal antibody against α-SMA. The percentage of α-SMA-positive cells to the total number of cells was determined. Results. Control gels cultured with MED 5 medium alone contracted to 15.8% ± 3.4% of their original area after 7 days. TGF- β2 significantly increased this contraction in a dose-dependent manner, whereas bFGF significantly decreased it. Approximately 30% of cells in the control gels were α-SMA positive. TGF-β2 significantly increased the α- SMA positivity dose dependently, whereas bFGF significantly decreased it. The percent positivity for α-SMA and the gel area showed a significant negative correlation. Conclusions. TGF-β2 increased collagen gel contraction and α- SMA expression in bovine LECs, whereas bFGF decreased these parameters. Because collagen gel contraction was correlated with α-SMA expression, the modulation of LEC contractile activity by growth factors may be related to an effect on α-SMA.

AB - Purpose. To determine whether basic fibroblast growth factor (bFGF) and transforming growth factor-β2 (TGF-β2) influence the contractile activity and the expression of α-smooth muscle actin (α-SMA) in bovine lens epithelial cells (LECs). To examine whether modulation of contractile activity by these growth factors depends on changes of α-SMA expression. Methods. Bovine LECs were cultured in collagen gel in MED 5 medium (F-12 nutrient mixture supplemented with 5% fatal bovine scrimp) with or without bFGF (1 to 100 ng/ml) or TGF-β2 (0.01 to 10 ng/ml). To evaluate collagen gel contraction, the longest and shortest diameters of the gels were measured daily for 7 days, and the area was determined. Detection of α-SMA in the gels was performed immunohistochemically using a mouse monoclonal antibody against α-SMA. The percentage of α-SMA-positive cells to the total number of cells was determined. Results. Control gels cultured with MED 5 medium alone contracted to 15.8% ± 3.4% of their original area after 7 days. TGF- β2 significantly increased this contraction in a dose-dependent manner, whereas bFGF significantly decreased it. Approximately 30% of cells in the control gels were α-SMA positive. TGF-β2 significantly increased the α- SMA positivity dose dependently, whereas bFGF significantly decreased it. The percent positivity for α-SMA and the gel area showed a significant negative correlation. Conclusions. TGF-β2 increased collagen gel contraction and α- SMA expression in bovine LECs, whereas bFGF decreased these parameters. Because collagen gel contraction was correlated with α-SMA expression, the modulation of LEC contractile activity by growth factors may be related to an effect on α-SMA.

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KW - basic fibroblast growth factor

KW - collagen gel

KW - lens epithelial cell

KW - transforming growth factor-beta

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