Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis

Nobuyoshi Akimitsu, Tohru Mizushima, Emi Suzuki, Takeyoshi Miki, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 μg/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype.

Original languageEnglish
Pages (from-to)1275-1278
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume19
Issue number10
DOIs
Publication statusPublished - 1996 Oct

Keywords

  • Escherichia coli
  • kanamycin
  • phosphatidylglycerol
  • temperature-sensitive growth

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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