Abstract
We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 μg/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype.
Original language | English |
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Pages (from-to) | 1275-1278 |
Number of pages | 4 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 19 |
Issue number | 10 |
DOIs | |
Publication status | Published - 1996 Oct |
Keywords
- Escherichia coli
- kanamycin
- phosphatidylglycerol
- temperature-sensitive growth
ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science