Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis

Nobuyoshi Akimitsu, Tohru Mizushima, Emi Suzuki, Takeyoshi Miki, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 μg/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype.

Original languageEnglish
Pages (from-to)1275-1278
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume19
Issue number10
Publication statusPublished - 1996 Oct
Externally publishedYes

Fingerprint

Phospholipids
Escherichia coli
Phenotype
Mutation
Growth
Phosphatidylglycerols
Plasmids
Kanamycin
Genes
Salts
Temperature

Keywords

  • Escherichia coli
  • kanamycin
  • phosphatidylglycerol
  • temperature-sensitive growth

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Akimitsu, N., Mizushima, T., Suzuki, E., Miki, T., & Sekimizu, K. (1996). Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis. Biological and Pharmaceutical Bulletin, 19(10), 1275-1278.

Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis. / Akimitsu, Nobuyoshi; Mizushima, Tohru; Suzuki, Emi; Miki, Takeyoshi; Sekimizu, Kazuhisa.

In: Biological and Pharmaceutical Bulletin, Vol. 19, No. 10, 10.1996, p. 1275-1278.

Research output: Contribution to journalArticle

Akimitsu, N, Mizushima, T, Suzuki, E, Miki, T & Sekimizu, K 1996, 'Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis', Biological and Pharmaceutical Bulletin, vol. 19, no. 10, pp. 1275-1278.
Akimitsu, Nobuyoshi ; Mizushima, Tohru ; Suzuki, Emi ; Miki, Takeyoshi ; Sekimizu, Kazuhisa. / Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis. In: Biological and Pharmaceutical Bulletin. 1996 ; Vol. 19, No. 10. pp. 1275-1278.
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