Abstract
We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 μg/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype.
| Original language | English |
|---|---|
| Pages (from-to) | 1275-1278 |
| Number of pages | 4 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 19 |
| Issue number | 10 |
| Publication status | Published - 1996 Oct |
| Externally published | Yes |
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Keywords
- Escherichia coli
- kanamycin
- phosphatidylglycerol
- temperature-sensitive growth
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis. / Akimitsu, Nobuyoshi; Mizushima, Tohru; Suzuki, Emi; Miki, Takeyoshi; Sekimizu, Kazuhisa.
In: Biological and Pharmaceutical Bulletin, Vol. 19, No. 10, 10.1996, p. 1275-1278.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Growth phenotypes of Escherichia coli carrying a mutation of acidic phospholipid synthesis
AU - Akimitsu, Nobuyoshi
AU - Mizushima, Tohru
AU - Suzuki, Emi
AU - Miki, Takeyoshi
AU - Sekimizu, Kazuhisa
PY - 1996/10
Y1 - 1996/10
N2 - We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 μg/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype.
AB - We identified novel phenotypes of a pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. The first phenotype is limited cell growth at a high temperature, under the condition of low salt. The phenotype was co-transduced with a phenotype lacking the potential to synthesize phosphatidylglycerol in a P1 transduction experiment, and was restored by transformation with a plasmid containing a wild type pgsA gene. The second phenotype of the pgsA3 mutant was resistant to growth in the presence of a low concentration of kanamycin (4 μg/ml). P1 transduction and transformation with the plasmid containing the wild-type pgsA gene revealed that the pgsA3 mutation was also responsible for the second phenotype.
KW - Escherichia coli
KW - kanamycin
KW - phosphatidylglycerol
KW - temperature-sensitive growth
UR - http://www.scopus.com/inward/record.url?scp=0029911939&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029911939&partnerID=8YFLogxK
M3 - Article
C2 - 8913496
AN - SCOPUS:0029911939
VL - 19
SP - 1275
EP - 1278
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 10
ER -